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This work presents a scalable workflow that imprints micro\u2010 and nano\u2010gratings into elastin\u2010like recombinamer (ELR)\u2010based hydrogels, enabling rapid endothelial cell capture and accelerating monolayer formation within 14 days. Three gelatin\u2010ELR formulations are engineered, with 1H\u2010NMR confirming incorporation of sequences designed to modulate bioactivity (ELR1: inert, ELR2: uPA\u2010responsive, ELR3: RGD\u2010adhesive). ELR incorporation generates fibrillar microstructures and enhances mechanical performance, yielding elastic\u2010dominant networks suitable for high\u2010fidelity pattern transfer. Using this library, the combined effects of ELR bioactivity and groove geometry on human iPSC\u2010derived endothelial cells are evaluated. In a 15\u2010min attachment assay, patterned ELR composites markedly improve cell retention compared to gelatin, with ELR2 on \u223c350 and \u223c4\u00a0\u00b5m grooves performing best, consistent with controlled, cell\u2010mediated interfacial remodeling. This early advantage persists, as ELR2 and ELR3 hydrogels support rapid alignment and reach confluence by day 14, whereas gelatin remains subconfluent. By combining enhanced early capture with protease\u2010regulated remodeling, ELR2 identifies a favorable design window. These results establish a materials design framework linking programmable ELR chemistry with surface topography to engineer endothelial interfaces, providing a versatile platform for vascular biomaterials and microphysiological systems.<\/jats:p>","DOI":"10.1002\/adhm.71570","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T05:35:57Z","timestamp":1786426557000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Patterned ELR\u2010Gelatin Hydrogels Enable Rapid Endothelial Monolayer Formation via Bioactive Matrix Chemistry and Surface Topography"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8515-2171","authenticated-orcid":false,"given":"Jagoda","family":"Litowczenko","sequence":"first","affiliation":[{"name":"Center For Advanced Technologies Adam Mickiewicz University in Pozna\u0144, Uniwersytetu Pozna\u0144skiego  Pozna\u0144 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L\u00b4Hospitalet De Llobregat  Barcelona Spain"},{"name":"Program For Clinical Translation of Regenerative Medicine in Catalonia (P\u2010[CMRC]) L\u00b4Hospitalet De Llobregat  Barcelona Spain"},{"name":"Center For Networked Biomedical Research On Bioengineering Biomaterials and Nanomedicine (CIBER\u2010BBN)  Madrid Spain"},{"name":"Institut De Microelectr\u00f2nica De Barcelona IMB\u2010CNM (CSIC), Bellaterra  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Uribe","sequence":"additional","affiliation":[{"name":"Stem Cell Potency Group, Regenerative Medicine Program Bellvitge Biomedical Research Institute (IDIBELL) L\u00b4Hospitalet De Llobregat  Barcelona Spain"},{"name":"Program For Clinical Translation of Regenerative Medicine in Catalonia (P\u2010[CMRC]) L\u00b4Hospitalet De Llobregat  Barcelona Spain"},{"name":"Center For Networked Biomedical Research On Bioengineering Biomaterials and Nanomedicine (CIBER\u2010BBN)  Madrid 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