{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T06:40:39Z","timestamp":1792737639707,"version":"4.5.0"},"reference-count":81,"publisher":"Wiley","issue":"39","license":[{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["465242756"],"award-info":[{"award-number":["465242756"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Meniscal injury is a leading cause of early\u2010onset osteoarthritis, yet regenerative options remain limited. This study investigates a nonwoven polyethylene terephthalate (PET) scaffold for meniscus tissue engineering and assesses its capacity to support mesenchymal stromal cell (MSC) proliferation and chondrogenic differentiation under dynamic loading. Human MSCs are seeded onto PET scaffolds (400\u2013420\u00a0g\/m\n                    <jats:sup>2<\/jats:sup>\n                    , 85% porosity) and cultured for up to 21 days under basal medium (Ctr), chondrogenic differentiation conditions (ChD), or ChD combined with dynamic loading (ChD\u00a0+\u00a0Dyn, 12% strain, 1\u00a0Hz, 1\u00a0h\/day, 5 days\/week). PET scaffolds support uniform MSC adhesion and colonization. Compared with ChD alone, ChD+Dyn significantly increases cell proliferation and transiently upregulated chondrogenic markers (\n                    <jats:italic>SOX9<\/jats:italic>\n                    ,\n                    <jats:italic>ACAN<\/jats:italic>\n                    ,\n                    <jats:italic>COL1A1<\/jats:italic>\n                    ,\n                    <jats:italic>COL2A1<\/jats:italic>\n                    ) while suppressing the hypertrophic marker\n                    <jats:italic>COL10A1<\/jats:italic>\n                    . Although collagen deposition and construct biomechanics remained unchanged over 21 days, glycosaminoglycan accumulation was reduced in the ChD\u00a0+\u00a0Dyn group compared with ChD. RNA sequencing revealed distinct mechanosensitive transcriptional signatures induced by dynamic loading, particularly in genes associated with extracellular matrix remodeling, mechanotransduction, and developmental signaling pathways. These findings demonstrate that nonwoven PET provides a mechanically robust scaffold for meniscus tissue engineering and that dynamic loading promotes MSC proliferation while transiently regulating chondrogenic differentiation and mechanoadaptive matrix remodeling toward a meniscus\u2010like phenotype.\n                  <\/jats:p>","DOI":"10.1002\/adhm.71574","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T05:39:58Z","timestamp":1786513198000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dynamic Loading Regulates Meniscus\u2010Like Matrix Production in Human Mesenchymal Stromal Cell\u2010Seeded PET Scaffolds"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4206-6326","authenticated-orcid":false,"given":"Graciosa Quelhas","family":"Teixeira","sequence":"first","affiliation":[{"name":"Institute of Orthopaedic Research and Biomechanics Ulm University Medical Center  Ulm Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6906-0599","authenticated-orcid":false,"given":"Luisa","family":"de Roy","sequence":"additional","affiliation":[{"name":"Institute of Orthopaedic Research and Biomechanics Ulm University Medical Center  Ulm Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-1558-7492","authenticated-orcid":false,"given":"Anna\u2010Lotta","family":"Feldmeier","sequence":"additional","affiliation":[{"name":"Institute of Orthopaedic Research and Biomechanics Ulm University Medical Center  Ulm Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9012-4039","authenticated-orcid":false,"given":"Mubashir","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Institute of Orthopaedic Research and Biomechanics Ulm University Medical Center  Ulm 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