{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T07:55:12Z","timestamp":1790668512715,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2022R1C1C1004803"],"award-info":[{"award-number":["2022R1C1C1004803"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Functional vascularization remains a critical challenge in the regeneration of full\u2010thickness skin defects. Here, we present a 3D embedded bioprinting strategy for engineering vascularized biopatches through the precise integration of prevascularized multicellular spheroids within a skin\u2010derived decellularized extracellular matrix (SdECM) bioink. Rheological results reveal that a 1.0% (w\/v) SdECM formulation exhibits yield\u2010stress behavior, pronounced shear\u2010thinning, and rapid shear recovery, enabling high\u2010fidelity of spheroids while preserving cell viability and structural integrity. Using this platform, endothelial spheroids with tunable diameters are fabricated to investigate size\u2010dependent vascular self\u2010organization. Intermediate\u2010sized spheroids (\u223c700\u00a0\u00b5m) demonstrate robust endothelial network formation, with enhanced vascular coverage, branching complexity, and angiogenic gene expression while avoiding excessive hypoxia. Spatial organization of prevascularized spheroids critically regulates paracrine\u2010mediated wound repair, with enhanced fibroblast migration observed when inter\u2010spheroid distances were within 1.8\u00a0mm. Furthermore, incorporation of mesenchymal stem cells (MSCs) provides perivascular\u2010like support, significantly stabilizing microvascular networks, with an optimized endothelial cell\u2013to\u2013MSC ratio of 1:0.5. In a full\u2010thickness murine wound model, multicellular spheroids\u2010laden SdECM biopatches significantly accelerate wound closure, enhance re\u2010epithelialization, promoted angiogenesis, and improve blood perfusion. Collectively, this study establishes a scalable embedded bioprinting for engineering prevascularized biopatches and provides design principles for spheroid\u2010based soft tissue regeneration.<\/jats:p>","DOI":"10.1002\/adhm.71616","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T05:26:48Z","timestamp":1787290008000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Spatially Controlled Embedded Bioprinting of Prevascularized Spheroids\u2010Containing Biopatch Enables Functional Skin Regeneration"],"prefix":"10.1002","author":[{"given":"Minjun","family":"Ahn","sequence":"first","affiliation":[{"name":"Medical Research Institute Pusan National University  Yangsan Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6649-8783","authenticated-orcid":false,"given":"Gyu Tae","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Physiology College of Medicine Pusan National University  Yangsan Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6828-401X","authenticated-orcid":false,"given":"Dongjun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Convergence Medicine School of Medicine Pusan National University  Yangsan Republic of Korea"},{"name":"Transplantation Research Center Research Institute for Convergence of Biomedical Science and Technology Pusan National University Yangsan Hospital  Yangsan Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6449-0761","authenticated-orcid":false,"given":"Sik","family":"Yoon","sequence":"additional","affiliation":[{"name":"Department of Anatomy School of Medicine Pusan National University  Yangsan Republic of 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University  Yangsan Republic of Korea"},{"name":"Department of Information Convergence Engineering Pusan National University  Busan Republic of Korea"},{"name":"School of Biomedical Convergence Engineering Pusan National University  Yangsan Republic of Korea"},{"name":"Research Institute for Convergence of Biomedical Science and Technology Pusan National University Yangsan Hospital  Yangsan Republic of 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