{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:00:53Z","timestamp":1790312453957,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"36","license":[{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFA1101903"],"award-info":[{"award-number":["2021YFA1101903"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Calcification remains a critical barrier to the long\u2010term durability of glutaraldehyde\u2010fixed decellularized bioprosthetic heart valves, despite their favorable biocompatibility and reduced thrombogenicity. Here, we identify polyaspartic acid (PASP) as a potent inhibitor of valve calcification. PASP modulates key pathological pathways by suppressing osteogenic differentiation (e.g., BMP2 and RUNX2), enhancing anti\u2010calcification regulators (e.g., OPN and MGP), and reducing apoptosis in vitro. In addition, its calcium\u2010chelating capability effectively inhibits dystrophic mineral deposition within the extracellular matrix in vivo. To achieve sustained and localized delivery of PASP, we developed a robust hydrogel coating on the valve surface via a surface\u2010catalyzed free\u2010radical polymerization reaction combined with UV\u2010mediated secondary photocrosslinking. This functional coating enables stable encapsulation and controlled release of PASP while preserving mechanical integrity and long\u2010term structural stability, thereby providing durable anti\u2010calcification performance. Collectively, our findings establish a bioinspired and clinically relevant strategy for preserving the structural and functional integrity of bioprosthetic heart valves and provide a generalizable platform for the design of anti\u2010calcification biomaterials for cardiovascular implants.<\/jats:p>","DOI":"10.1002\/adhm.71626","type":"journal-article","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T04:52:36Z","timestamp":1787633556000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Polyaspartic Acid\u2010Doped With Robust Polymerized Hydrogel Armor Enhances Anti\u2010Calcification of Decellularized Porcine Heart Valves"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4542-4275","authenticated-orcid":false,"given":"Jinyu","family":"Li","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7519-2907","authenticated-orcid":false,"given":"Mengxue","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yin","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery Union Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyi","family":"Wan","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery Union Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruiyue","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3867-6607","authenticated-orcid":false,"given":"Zhiqiang","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5437-599X","authenticated-orcid":false,"given":"Nianguo","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery Union Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0733-2097","authenticated-orcid":false,"given":"Jianglin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Huazhong University of Science and Technology  Wuhan China"},{"name":"Shenzhen Huazhong University of Science and Technology Research Institute  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,24]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41569\u2010018\u20100119\u20104"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41569\u2010021\u201000570\u2010z"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIR.0000000000001123"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(23)02754\u2010X"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202315826"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2009.10.085"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201700918"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2021.09.014"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202309844"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvac002"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehab757"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.120.018506"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41569\u2010020\u20100422\u20108"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.124.071954"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(23)01518\u20100"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41569\u2010023\u201000845\u20107"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1161\/circ.144.suppl_1.12316"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.123.066523"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.71813"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2023.02.009"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40824\u2010023\u201000348\u2010z"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/14728222.2022.2118576"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204\u2010021\u201003220\u2010y"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202309042"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmm2.12026"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2024.01.006"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2024.06.047"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/nsr\/nwaa254"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201052023\u2010z"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xcrm.2025.102019"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(16)00436\u20100"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41578\u2010021\u201000405\u2010w"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xcrm.2025.101987"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202108889"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2007.12.005"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/9783527619443.ch16"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00395\u2010021\u201000899\u2010z"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.01.057"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.compositesb.2021.109504"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2021.128900"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202500856"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells15060542"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40712-025-00263-2"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.31083\/RCM36688"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/rb\/rbae003"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202504133"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202506728"}],"container-title":["Advanced Healthcare Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.71626","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/adhm.71626","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.71626","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T04:45:33Z","timestamp":1790311533000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adhm.71626"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,24]]},"references-count":47,"journal-issue":{"issue":"36","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1002\/adhm.71626"],"URL":"https:\/\/doi.org\/10.1002\/adhm.71626","archive":["Portico"],"relation":{},"ISSN":["2192-2640","2192-2659"],"issn-type":[{"value":"2192-2640","type":"print"},{"value":"2192-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,24]]},"assertion":[{"value":"2026-03-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71626"}}