{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:00:47Z","timestamp":1790312447001,"version":"4.1.0"},"reference-count":66,"publisher":"Wiley","issue":"36","license":[{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","award":["101075873"],"award-info":[{"award-number":["101075873"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Diseases of the ocular surface, such as dry eye disease (DED), can significantly impair vision and reduce the quality of life. Current treatments rely primarily on conventional approaches, including artificial tears, antibiotics, and anti\u2010inflammatory drugs. However, advances in nucleic acid (NA) therapeutics have opened new avenues for treating ocular surface disorders, with growing interest in small interfering RNA (siRNA) and messenger RNA (mRNA) as potential drugs. A key challenge remains the efficient delivery of these macromolecules into cells of the ocular surface, where both extracellular and intracellular barriers hinder their cellular uptake and activity. To address this limitation, we explore the use of photoporation, a laser\u2010based physical transfection technique, to enhance mRNA delivery into ocular surface cells. Photoporation employs pulsed\u2010laser irradiation of photosensitizers to generate vapor nanobubbles (VNBs), whose rapid expansion and implosion produce mechanical forces that transiently permeabilize cell membranes, thereby promoting intracellular delivery of co\u2010administered nucleic acids. Using polydopamine nanoparticles (PD NPs) as biocompatible photosensitizers for photoporation, we demonstrate mRNA transfection in the corneal epithelium with both an experimental and a clinically approved pulsed\u2010laser system. Our results highlight the potential of laser\u2010based approaches for noninvasive engineering of the ocular surface.<\/jats:p>","DOI":"10.1002\/adhm.71643","type":"journal-article","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T04:55:05Z","timestamp":1787633705000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Light\u2010Mediated Transfection of the Ocular Surface Using an Ophthalmic YAG Laser"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2087-2781","authenticated-orcid":false,"given":"Junho","family":"Byun","sequence":"first","affiliation":[{"name":"Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences Ghent University  Ghent Belgium"},{"name":"Research Institute of Pharmaceutical Sciences, College of Pharmacy Sookmyung Women's University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mike","family":"Wels","sequence":"additional","affiliation":[{"name":"Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences Ghent University  Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8369-7282","authenticated-orcid":false,"given":"Bernd","family":"Vanmeerhaeghe","sequence":"additional","affiliation":[{"name":"Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences Ghent University  Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4252-7156","authenticated-orcid":false,"given":"Luca","family":"Paoletti","sequence":"additional","affiliation":[{"name":"Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences Ghent University  Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0002-390X","authenticated-orcid":false,"given":"Tao","family":"Lu","sequence":"additional","affiliation":[{"name":"Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences Ghent University  Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aranit","family":"Harizaj","sequence":"additional","affiliation":[{"name":"Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences Ghent University  Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-5266-7585","authenticated-orcid":false,"given":"Jonathan","family":"Tavernier","sequence":"additional","affiliation":[{"name":"Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences Ghent University  Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0051-0855","authenticated-orcid":false,"given":"Dimitri","family":"Roels","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Ghent University Hospital  Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilia","family":"Goemaere","sequence":"additional","affiliation":[{"name":"Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences Ghent University  Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2244-1339","authenticated-orcid":false,"given":"Katrien","family":"Remaut","sequence":"additional","affiliation":[{"name":"Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences Ghent University  Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9807-4715","authenticated-orcid":false,"given":"Koen","family":"Raemdonck","sequence":"additional","affiliation":[{"name":"Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences Ghent University  Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7993-6295","authenticated-orcid":false,"given":"Kevin","family":"Braeckmans","sequence":"additional","affiliation":[{"name":"Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences Ghent University  Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8653-2598","authenticated-orcid":false,"given":"Stefaan C.","family":"De Smedt","sequence":"additional","affiliation":[{"name":"Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences Ghent University  Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8065-4439","authenticated-orcid":false,"given":"F\u00e9lix","family":"Sauvage","sequence":"additional","affiliation":[{"name":"Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences Ghent University  Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,24]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtos.2017.05.003"},{"key":"e_1_2_12_3_1","first-page":"163","article-title":"Persistent Corneal Epithelial Defects: A Review Article","volume":"8","author":"Vaidyanathan U.","year":"2019","journal-title":"Medical Hypothesis\u2013 Discovery & Innovation in Ophthalmology"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2025.113811"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2025.113681"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2025.01.068"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/13543784.2018.1457647"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/humu.22764"},{"key":"e_1_2_12_9_1","first-page":"1378","article-title":"Identification of Presumed Pathogenic KRT3 and KRT12 Gene Mutations Associated With Meesmann Corneal Dystrophy","volume":"21","author":"Chen J. L.","year":"2015","journal-title":"Molecular Vision"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2024.115317"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201080542\u20104"},{"key":"e_1_2_12_12_1","unstructured":"S. A.Sylentis Safety Study of Tivanisiran to Treat Dry Eye clinicaltrialsgov 2024 https:\/\/clinicaltrials.gov\/study\/NCT05310422(accessed April 14 2025)."},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1089\/jop.1987.3.159"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1018938310628"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.EJPS.2018.03.034"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/GB\u20102006\u20107\u20108\u2010R72"},{"key":"e_1_2_12_17_1","first-page":"180","article-title":"Tear Lipocalin is the Major Endonuclease in Tears","volume":"14","author":"Yusifov T. N.","year":"2008","journal-title":"Molecular Vision"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1039\/D3CS00194F"},{"key":"e_1_2_12_19_1","first-page":"264","article-title":"Determination of Tear Volume and Tear Flow","volume":"5","author":"Mishima S.","year":"1966","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"e_1_2_12_20_1","doi-asserted-by":"crossref","unstructured":"A.Popov \u201cMucus\u2010Penetrating Particles and the Role of Ocular Mucus as a Barrier to Micro\u2010 and Nanosuspensions \u201dJournal of Ocular Pharmacology and Therapeutics36(2020):366\u2013375 jop.2020.0022 https:\/\/doi.org\/10.1089\/jop.2020.0022.","DOI":"10.1089\/jop.2020.0022"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/ACI.0b013e32830e6b04"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.EJPB.2015.02.032"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.IJPHARM.2017.09.018"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/PHARMACEUTICS10010028"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2021.04.008"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0172928"},{"key":"e_1_2_12_27_1","unstructured":"admin AAV VECTOR MANUFACTURING\u2014Challenges & Opportunities in the Manufacturing of AAV Vectors Used in the Delivery of Gene Therapy Treatments Drug Dev Deliv (2017) https:\/\/drug\u2010dev.com\/aav\u2010vector\u2010manufacturing\u2010challenges\u2010opportunities\u2010in\u2010the\u2010manufacturing\u2010of\u2010aav\u2010vectors\u2010used\u2010in\u2010the\u2010delivery\u2010of\u2010gene\u2010therapy\u2010treatments\/(accessed April 14 2025)."},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpb.2016.10.009"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727\u2010232\u20102320362"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/jgm.231"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161\u20106420(98)91129\u20100"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.survophthal.2003.08.002"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaophthalmol.2017.2388"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/NN5017742\/SUPPL_FILE\/NN5017742_SI_001.PDF"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.JCONREL.2017.08.002"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/S40820\u2010020\u201000523\u20100\/FIGURES\/7"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/IJMS20174254"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.COCIS.2021.101453"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.00971"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/ADFM.202102472"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2013.07.027"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-30406-4_19"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.198119"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1364\/OE.17.002538"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpcc.8b07672"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpcc.5b09903"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.9b04050"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.2217\/nnm.09.59"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/CRYST9080411"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010018\u201006884\u2010w"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.157.3.9703059"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41377\u2010018\u20100048\u20103"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.JCONREL.2017.09.033"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.1021\/ACS.NANOLETT.6B01411"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.1021\/ACS.NANOLETT.6B00562"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.JCONREL.2011.02.006"},{"key":"e_1_2_12_57_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.84.11.1255"},{"key":"e_1_2_12_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202008379"},{"key":"e_1_2_12_59_1","doi-asserted-by":"publisher","DOI":"10.3390\/polym12071519"},{"key":"e_1_2_12_60_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10103\u2010024\u201004118\u20108"},{"key":"e_1_2_12_61_1","doi-asserted-by":"publisher","DOI":"10.2147\/OPTH.S53490"},{"key":"e_1_2_12_62_1","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.390849"},{"key":"e_1_2_12_63_1","unstructured":"Tango Reflex Multi\u2010Modality YAG\/SLT Laser (n.d.) https:\/\/media.lumibirdmedicalnordics.se\/2021\/09\/PDF.PageParPage.TangoReflex.pdf."},{"key":"e_1_2_12_64_1","doi-asserted-by":"publisher","DOI":"10.1002\/1096\u20109101(2000)27:5<451::AID\u2010LSM1006>3.0.CO;2\u20101"},{"key":"e_1_2_12_65_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm101281b"},{"key":"e_1_2_12_66_1","doi-asserted-by":"publisher","DOI":"10.1201\/9781420078893-c5"},{"key":"e_1_2_12_67_1","doi-asserted-by":"publisher","DOI":"10.1167\/IOVS.13\u201011717"}],"container-title":["Advanced Healthcare Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.71643","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/adhm.71643","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.71643","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T04:46:24Z","timestamp":1790311584000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adhm.71643"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,24]]},"references-count":66,"journal-issue":{"issue":"36","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1002\/adhm.71643"],"URL":"https:\/\/doi.org\/10.1002\/adhm.71643","archive":["Portico"],"relation":{},"ISSN":["2192-2640","2192-2659"],"issn-type":[{"value":"2192-2640","type":"print"},{"value":"2192-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,24]]},"assertion":[{"value":"2026-04-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71643"}}