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Hair follicle morphogenesis relies on precisely coordinated epithelial\u2010mesenchymal interactions (EMIs) within a spatially defined epidermal\u2013dermal niche, which is difficult to reconstruct using conventional tissue engineering approaches. We present a modular tissue engineering strategy using a self\u2010healing\n                    <jats:italic>N<\/jats:italic>\n                    \u2010carboxyethyl chitosan\u2010oxidized dextran\/collagen\u2013genipin hydrogel. The hydrogel exhibits tunable mechanical properties, achieving a mechanically permissive stiffness conducive for hair follicle inductivity (\u223c32\u00a0kPa), and enables the bottom\u2010up assembly of multicellular hair\u2010follicle mimetic tissues. Two independently engineered hydrogel modules, one encapsulating dermal papilla\u2010keratinocyte spheroids filled with singlet keratinocytes and the other containing a dermal fibroblast, spontaneously fuse vertically through self\u2010healing to form a bilayered epidermal\u2013dermal construct. This spatially organized architecture promotes EMIs, enhances folliculogenic signaling, supports in vitro follicle\u2010like morphogenesis, and reproduces pharmacological responses to clinically approved anti\u2010alopecia drugs. In vivo implantation of the bilayered construct accelerates wound healing and promotes de novo hair follicle regeneration, with the implanted human cells contributing to this process. This study establishes a scalable and clinically translatable approach tailored for personalized hair regeneration therapy and the high\u2010throughput pharmacological screening of anti\u2010alopecia drug candidates.\n                  <\/jats:p>","DOI":"10.1002\/adhm.71722","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T05:44:17Z","timestamp":1789451057000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Self\u2010Healing Hydrogel\u2010Enabled Modular Assembly of Bilayered Skin Construct for Hair Follicle Regeneration"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-7546-6852","authenticated-orcid":false,"given":"JaeWook","family":"Park","sequence":"first","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering Yonsei University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Su Yong","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery Chung\u2010Ang University Hospital Chung\u2010Ang University College of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MinSu","family":"Jeong","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering Yonsei University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2320-7621","authenticated-orcid":false,"given":"Beom Joon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Dermatology Chung\u2010Ang University Hospital Chung\u2010Ang University College of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shin Hyuk","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery Chung\u2010Ang University Hospital Chung\u2010Ang University College of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5191-2531","authenticated-orcid":false,"given":"Won\u2010Gun","family":"Koh","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering Yonsei University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,14]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1473\u20102165.2005.00158.x"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S542000"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12020\u2010017\u20101280\u2010y"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0190\u20109622(92)70134\u20102"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003495\u2010198122040\u201000001"},{"key":"e_1_2_10_7_1","first-page":"56","article-title":"Adverse Effects and Safety of 5\u2010alpha Reductase Inhibitors (finasteride, dutasteride): a Systematic Review","volume":"9","author":"Hirshburg J. 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