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Here, we introduce a scalable and versatile biofabrication platform that enables the controlled assembly of multitype spheroid arrays (MSA) within standard culture microplates. By performing programmable bioprinting within soft, microstructured culture devices, we achieve the precise spatial deposition of multiple cell types into defined microwells, yielding reproducible arrays of heterogeneous 3D tumor spheroids that collectively recapitulate the macro\u2010 and micro\u2010heterogeneity of solid cancers. We demonstrate that this approach permits the fine\u2010tuning of spheroid size, composition, and arrangement. It also supports the delayed secondary programmed seeding of cells on pre\u2010grown spheroids, mimicking biological processes such as the invasion of immune cells. These methods enable quantitative drug\u2010response profiling within a single assay. They overcome some critical limitations of current 3D cancer models, introducing the concept of the \u201ccancer\u2010in\u2010a\u2010well\u201d technology. This technology provides a simple\u2010to\u2010use, cost\u2010effective, high\u2010throughput, and highly reproducible platform to recreate the complexity of tumors in vitro using methods and instruments that can be potentially used by any researcher in any cell culture lab. We can foresee a positive impact on cancer biology studies, drug screening, and personalized oncology.<\/jats:p>","DOI":"10.1002\/adhm.71725","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T04:12:05Z","timestamp":1789963925000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Hybrid Bioprinting\u2013Microfabrication Approach to Controllable Multitype Spheroid Arrays for Modeling Tumor Heterogeneity"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6341-4185","authenticated-orcid":false,"given":"Simone","family":"Bonetti","sequence":"first","affiliation":[{"name":"Institute for the Study of Nanostructured Materials National Research Council of Italy  Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7344-2529","authenticated-orcid":false,"given":"Eva","family":"Clemente","sequence":"additional","affiliation":[{"name":"Institute for the Study of Nanostructured Materials National Research Council of Italy  Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3284-6161","authenticated-orcid":false,"given":"Emanuela","family":"Mens\u00e0","sequence":"additional","affiliation":[{"name":"Department of Pharmacy and Biotechnology University of Bologna  Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2979-1383","authenticated-orcid":false,"given":"Marco","family":"de Maria","sequence":"additional","affiliation":[{"name":"Department of Pharmacy and Biotechnology University of Bologna  Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6254-9443","authenticated-orcid":false,"given":"Fulvia","family":"Farabegoli","sequence":"additional","affiliation":[{"name":"Department of Pharmacy and Biotechnology University of Bologna  Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9140-3128","authenticated-orcid":false,"given":"Emanuela","family":"Saracino","sequence":"additional","affiliation":[{"name":"Institute for the Organic Synthesis and Photoreactivity National Research Council of Italy  Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1240-9391","authenticated-orcid":false,"given":"Barbara","family":"Bortolani","sequence":"additional","affiliation":[{"name":"eDIMES Lab \u2013 DIMEC, Policlinico S. 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