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Here, we developed a dual E\u2010\/VE\u2010cadherin\u2010Fc (EVE) interface to condition human mesenchymal stem cells (MSCs) under CTGF\/TGF\u2010\u03b2 stimulation. Cells showed increased expression of Gli\u20101, NG2, PDGFR\u03b2, and FAP and activated transcription of extracellular matrix remodeling. These changes were consistent with the acquisition of a Gli\u20101\n                    <jats:sup>+<\/jats:sup>\n                    perivascular\u2010like profibrotic stromal phenotype, and were accompanied by reorganization of cadherin\u2010catenin complexes, actin cytoskeletal rearrangement, and increased YAP nuclear localization. Cell\u2010sized PLGA\/chitosan\u2010heparin microparticles were subsequently functionalized with E\u2010\/VE\u2010cadherin\u2010Fc and loaded with CTGF\/TGF\u2010\u03b2. Co\u2010assembly of these microparticles with the conditioned stromal cells (gMSCs) and renal epithelial cells (HK\u20102) generated 3D renal fibrotic microtissues that combined cadherin\u2010mediated adhesive presentation with localized cytokine delivery. The resulting microtissues exhibited a broader distribution of \u03b1\u2010SMA, Collagen I, and Fibronectin, together with reciprocal epithelial\u2010stromal signaling and spatially organized fibrotic activation. As a proof of concept for pharmacological evaluation, the microtissues responded to pirfenidone (PFD) in a dose\u2010dependent manner, with 100\u00a0\u00b5M PFD reducing \u03b1\u2010SMA expression by \u223c73% while preserving microtissue viability. Together, these findings establish a material\u2010enabled, cadherin\u2010guided strategy for constructing disease\u2010relevant stromal organization and spatially controlled profibrotic signaling in human renal fibrosis models.\n                  <\/jats:p>","DOI":"10.1002\/adhm.71744","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T03:53:26Z","timestamp":1789962806000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["E\u2010\/VE\u2010Cadherin\u2010Fc Interface Programs Gli\u20101\n                    <sup>+<\/sup>\n                    Stromal Cells for Assembly With Functionalized Microparticles Into 3D Renal Fibrotic Microtissues for Drug Screening"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-2793-1450","authenticated-orcid":false,"given":"Zhong","family":"Cheng","sequence":"first","affiliation":[{"name":"The Key Laboratory of Bioactive Materials 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