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Although electroacupuncture (EA) shows clinical potential, its cellular mechanisms in tendon healing are not fully understood. Here, we integrated publicly available single\u2010cell RNA sequencing datasets with bulk transcriptomic profiling from a murine Achilles tendinopathy model to establish a tendon cellular reference atlas and characterize MMP2\n                    <jats:sup>+<\/jats:sup>\n                    fibroblast\u2010associated ECM remodeling states, including High_ECM and Low_ECM programs. EA significantly improves pain sensitivity, locomotor function, and collagen organization while reducing fibrotic adhesions. Transcriptomic analysis reveals that EA reverses inflammatory and matrix\u2010degradation pathways and upregulates collagen\u2010assembly and ECM\u2013receptor interaction genes. Integrated single\u2010cell reference mapping and bulk transcriptomic analysis revealed that EA\u2010associated tendon repair was accompanied by enrichment of ECM\u2010related transcriptional programs overlapping with MMP2\n                    <jats:sup>+<\/jats:sup>\n                    fibroblast\u2010associated ECM remodeling states. Single\u2010cell co\u2010expression analysis identified coordinated expression patterns of key ECM\u2010related genes. Our study suggests that EA\u2010associated tendon repair is accompanied by modulation of ECM\u2010related molecular programs overlapping with fibroblast\u2010associated ECM remodeling states, providing new insights into the molecular framework underlying tendon repair after ECM injury.\n                  <\/jats:p>","DOI":"10.1002\/adhm.71774","type":"journal-article","created":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T10:48:17Z","timestamp":1791283697000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Single\u2010Cell Reference Mapping and Transcriptomic Analysis Identify Fibroblast\u2010Related ECM Remodeling During Electroacupuncture Treatment for Tendon Repair"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4354-8602","authenticated-orcid":false,"given":"Yang","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Sports Medicine Peking University Third Hospital, Institute of Sports Medicine of Peking University  Beijing China"},{"name":"Beijing Key Laboratory of Research and Translation for Drugs and Medical Devices in Precision Diagnosis and Treatment of Sports Injuries  Beijing China"},{"name":"Engineering Research Center of Sports Trauma Treatment Technology and Devices\uff0cMinistry of Education  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boyun","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine Peking University Third Hospital, Institute of Sports Medicine of Peking University  Beijing China"},{"name":"Beijing Key Laboratory of Research and Translation for Drugs and Medical Devices in Precision Diagnosis and Treatment of Sports Injuries  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zitian","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine Peking University Third Hospital, Institute of Sports Medicine of Peking University  Beijing China"},{"name":"Beijing Key Laboratory of Research and Translation for Drugs and Medical Devices in Precision Diagnosis and Treatment of Sports Injuries  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0714-0283","authenticated-orcid":false,"given":"Yucheng","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine Peking University Third Hospital, Institute of Sports Medicine of Peking University  Beijing China"},{"name":"Beijing Key Laboratory of Research and Translation for Drugs and Medical Devices in Precision Diagnosis and Treatment of Sports Injuries  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinrui","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine Peking University Third Hospital, Institute of Sports Medicine of Peking University  Beijing China"},{"name":"Beijing Key Laboratory of Research and Translation for Drugs and Medical Devices in Precision Diagnosis and Treatment of Sports Injuries  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine Peking University Third Hospital, Institute of Sports Medicine of Peking University  Beijing China"},{"name":"Beijing Key Laboratory of Research and Translation for Drugs and Medical Devices in Precision Diagnosis and Treatment of Sports Injuries  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenghui","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine Peking University Third Hospital, Institute of Sports Medicine of Peking University  Beijing China"},{"name":"Beijing Key Laboratory of Research and Translation for Drugs and Medical Devices in Precision Diagnosis and Treatment of Sports Injuries  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine Peking University Third Hospital, Institute of Sports Medicine of Peking University  Beijing China"},{"name":"Beijing Key Laboratory of Research and Translation for Drugs and Medical Devices in Precision Diagnosis and Treatment of Sports Injuries  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5724-2375","authenticated-orcid":false,"given":"Jin","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine Peking University Third Hospital, Institute of Sports Medicine of Peking University  Beijing China"},{"name":"Beijing Key Laboratory of Research and Translation for Drugs and Medical Devices in Precision Diagnosis and Treatment of Sports Injuries  Beijing China"},{"name":"Engineering Research Center of Sports Trauma Treatment Technology and Devices\uff0cMinistry of Education  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0504-7169","authenticated-orcid":false,"given":"Jianquan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine Peking University Third Hospital, Institute of Sports Medicine of Peking University  Beijing China"},{"name":"Beijing Key Laboratory of Research and Translation for Drugs and Medical Devices in Precision Diagnosis and Treatment of Sports Injuries  Beijing China"},{"name":"Engineering Research Center of Sports Trauma Treatment Technology and Devices\uff0cMinistry of Education  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongjie","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine Peking University Third Hospital, Institute of Sports Medicine of Peking University  Beijing China"},{"name":"Beijing Key Laboratory of Research and Translation for Drugs and Medical Devices in Precision Diagnosis and Treatment of Sports Injuries  Beijing China"},{"name":"Engineering Research Center of Sports Trauma Treatment Technology and Devices\uff0cMinistry of Education  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,6]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s12276\u2010024\u201001183\u20105"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2017.7380"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13075\u2010016\u20101218\u20104"},{"key":"e_1_2_11_5_1","doi-asserted-by":"crossref","DOI":"10.1002\/advs.202519568","article-title":"Advancing the Tendinopathy Triad: Comment on \u2018Tendinopathy: The Interplay Between Mechanical Stress, Inflammation, and Vascularity\u2019","volume":"13","author":"Yang D.","year":"2025","journal-title":"Advanced Science"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572\u2010021\u201000251\u20108"},{"key":"e_1_2_11_7_1","doi-asserted-by":"crossref","DOI":"10.1155\/2022\/2799665","article-title":"Pathological Tendon Histology in Early and Chronic Human Patellar Tendinopathy","volume":"2022","author":"Malmgaard\u2010Clausen N. 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Root Ganglia of Rats With Knee Osteoarthritis]","volume":"45","author":"Yang K. 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