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Here, we develop epitope\u2010imprinted polymer nanoparticles (nanoMIPs) for vimentin recognition through a two\u2010step strategy comprising screening a library of non\u2010imprinted nanoparticles to identify a lead formulation, followed by peptide\u2010directed molecular imprinting. The resulting nanoMIPs exhibited enhanced apparent affinity for vimentin, with picomolar\u2010range\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>D<\/jats:sub>\n                    values, although these values represent model\u2010dependent ensemble apparent affinities that may include contributions from multiple nanoparticle\u2010ligand contacts and local rebinding rather than intrinsic monovalent affinities. The nanoMIPs preferentially bound cancer cells presenting cell\u2010surface vimentin in vitro. The enhanced target binding and reduced off\u2010target binding were consistent with an imprinting\u2010associated improvement in molecular discrimination, although nonspecific physicochemical interactions were not completely eliminated. These findings extend epitope\u2010guided nanoMIP design to structurally dynamic, cell surface\u2010associated cytoskeletal targets, and support the development of synthetic receptors for tumor imaging. In vivo, the optimized nanoMIPs produced a tumor\u2010associated fluorescence signal approximately 1.44 times that of the matched non\u2010imprinted nanoparticles.\n                  <\/jats:p>","DOI":"10.1002\/adhm.71813","type":"journal-article","created":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:29:38Z","timestamp":1791275378000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Epitope\u2010Imprinted Polymer Nanoparticles Enable Selective Recognition of Filamentous Vimentin for Tumor Imaging In Vivo"],"prefix":"10.1002","author":[{"given":"Kamaran Khurshid","family":"Dar","sequence":"first","affiliation":[{"name":"State Key Laboratory of Organic\u2010Inorganic Composites Beijing University of Chemical Technology  Beijing China"},{"name":"National Energy R&amp;D Center for Biorefinery, International Joint Bioenergy Laboratory of Ministry of Education, College of Life Science and Technology Beijing University of Chemical Technology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2022-6595","authenticated-orcid":false,"given":"Yuan","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Organic\u2010Inorganic Composites Beijing University of Chemical Technology  Beijing China"},{"name":"National Energy R&amp;D Center for Biorefinery, International Joint Bioenergy Laboratory of Ministry of Education, College of Life Science and Technology Beijing University of Chemical Technology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9030-8006","authenticated-orcid":false,"given":"Yongqin","family":"Lv","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Organic\u2010Inorganic Composites Beijing University of Chemical Technology  Beijing China"},{"name":"National Energy R&amp;D Center for Biorefinery, International Joint Bioenergy Laboratory of Ministry of Education, College of Life Science and Technology Beijing University of Chemical Technology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,6]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010023\u201001366\u2010y"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202314262"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202004764"},{"key":"e_1_2_11_5_1","doi-asserted-by":"crossref","first-page":"11935","DOI":"10.1021\/acsnano.3c04017","article-title":"Enzyme Regulating the Wettability of the Outer Surface of Nanochannels","volume":"17","author":"Hu J. 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