{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T10:56:31Z","timestamp":1791543391914,"version":"4.3.4"},"reference-count":60,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:00:00Z","timestamp":1791504000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:00:00Z","timestamp":1791504000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Chronic wounds represent a burden for healthcare systems and critically impair quality of life of patients. Engineered living materials (ELMs) show great potential as scaffolds and delivery systems in tissue regeneration. As a generally regarded safe biofilm\u2010producing organism,\n                    <jats:italic>Bacillus subtilis<\/jats:italic>\n                    (\n                    <jats:italic>B. subtilis<\/jats:italic>\n                    ) presents interesting properties to develop functional ELMs in biomedicine. In this study, we engineer\n                    <jats:italic>B. subtilis<\/jats:italic>\n                    to design a protein\u2010based living matrix eluting bioactive sequences for wound healing. Accordingly, we create a biofilm\u2010deficient\n                    <jats:italic>B. subtilis<\/jats:italic>\n                    strain that expressed the biofilm scaffold protein, TasA in fusion with a cell\u2010adhesive motif (TasA\u2010PRb), which results in biofilms with cell\u2010adhesive properties for fibroblasts and keratinocytes. Besides,\n                    <jats:italic>B. subtilis<\/jats:italic>\n                    is known to elicit antimicrobial properties against strains of\n                    <jats:italic>E. coli<\/jats:italic>\n                    and\n                    <jats:italic>S. epidermidis<\/jats:italic>\n                    , which are not impeded by biofilm engineering. We also incorporate secretion of fibroblast growth factor 2 (FGF2), known to promote wound healing, in the biofilm\u2010deficient strain. Co\u2010culture of FGF2\u2010expressing and TasA\u2010PRb producing strains resulted in biofilms secreting bioactive FGF2. Chronic wound healing assays in diabetic mice reveal accelerated wound closure in mice treated with the TasA\u2010PRb\/FGF2 biofilms, with absence of observable adverse effects. This underlines the potential of the\n                    <jats:italic>B. subtilis<\/jats:italic>\n                    biofilm for the design of functional ELMs in biomedicine.\n                  <\/jats:p>","DOI":"10.1002\/adhm.71823","type":"journal-article","created":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T10:34:58Z","timestamp":1791542098000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["An Antimicrobial, Cell\u2010Adhesive, and Growth Factor\u2010Producing Living Material Engineered From\n                    <i>Bacillus subtilis<\/i>\n                    Biofilms for Wound Healing"],"prefix":"10.1002","author":[{"given":"M\u00e9lanie","family":"C\u00f4t\u00e9\u2010Cyr","sequence":"first","affiliation":[{"name":"Department of Chemistry Universit\u00e9 Du Qu\u00e9bec \u00e0 Montr\u00e9al (UQAM)  Montr\u00e9al Canada"},{"name":"Quebec Network for Research on Protein Function Engineering and Applications (PROTEO)  Montreal Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie\u2010Jeanne","family":"Archambault","sequence":"additional","affiliation":[{"name":"Department of Chemistry Universit\u00e9 Du Qu\u00e9bec \u00e0 Montr\u00e9al (UQAM)  Montr\u00e9al Canada"},{"name":"Quebec Network for Research on Protein Function Engineering and Applications (PROTEO)  Montreal Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Dupuis","sequence":"additional","affiliation":[{"name":"Department of Chemistry Universit\u00e9 Du Qu\u00e9bec \u00e0 Montr\u00e9al (UQAM)  Montr\u00e9al Canada"},{"name":"Quebec Network for Research on Protein Function Engineering and Applications (PROTEO)  Montreal 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