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In this study, we developed a straightforward hydrothermal method to synthesize\n                    <jats:italic>Astragalus membranaceus<\/jats:italic>\n                    \u2010derived carbon dots (AM\u2010CDs), using this medicinal herb, known for its cardiovascular protective effects, as a biomass precursor. The resulting AM\u2010CDs exhibited broad\u2010spectrum ROS\u2010scavenging activity and pronounced biological effects against oxidative stress, inflammation, and apoptosis. In vitro experiments revealed that AM\u2010CDs restored mitochondrial membrane potential and reduced DNA damage, as indicated by decreased \u03b3\u2010H2AX expression. Furthermore, AM\u2010CDs downregulated pro\u2010inflammatory cytokine mRNA expression in LPS\u2010stimulated macrophages. In a rat model of MIRI, direct intramyocardial administration of AM\u2010CDs resulted in a significant reduction in infarct size, improved left ventricular ejection fraction and fractional shortening in a dose\u2010dependent manner, and exhibited no notable systemic toxicity. Transcriptomic and protein analyses further demonstrated that AM\u2010CDs downregulate pro\u2010apoptotic proteins and modulate the PI3K\u2010AKT and NF\u2010\u03baB\u2010apoptosis signaling pathways, thereby disrupting the vicious cycle of oxidative stress, inflammation, and cell death. Collectively, these findings indicate that AM\u2010CDs offer a promising therapeutic approach that integrates traditional medicine with nanotechnology to provide effective cardioprotection against MIRI.\n                  <\/jats:p>","DOI":"10.1002\/adhm.71833","type":"journal-article","created":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T06:16:18Z","timestamp":1791872178000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>Astragalus membranaceus<\/i>\n                    \u2010Derived Carbon Dots Nanozymes Ameliorate Myocardial Ischemia\u2013Reperfusion Injury via Combined Pharmacological Action and Antioxidant Catalysis"],"prefix":"10.1002","volume":"15","author":[{"given":"Yapeng","family":"Guo","sequence":"first","affiliation":[{"name":"Department of Pharmacology School of Basic Medical Sciences Xi'an Jiaotong University  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhichao","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Basic Medical Sciences Xi'an Jiaotong University  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Basic Medical Sciences Xi'an Jiaotong University  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenxi","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Basic Medical Sciences Xi'an Jiaotong University  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bowen","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Basic Medical Sciences Xi'an Jiaotong University  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7283-5933","authenticated-orcid":false,"given":"Junlong","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Basic Medical Sciences Xi'an Jiaotong University  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujie","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Basic Medical Sciences Xi'an Jiaotong University  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2159-9190","authenticated-orcid":false,"given":"Jian","family":"Song","sequence":"additional","affiliation":[{"name":"Institute of Cardiovascular Sciences Guangxi Academy of Medical Sciences The People's Hospital of Guangxi Zhuang Autonomous Region  Nanning Guangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lianying","family":"Jiao","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Basic Medical Sciences Xi'an Jiaotong University  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4686-6526","authenticated-orcid":false,"given":"Mingzhen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Basic Medical Sciences Xi'an Jiaotong University  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/nejmoa0908610"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10147"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/nejmra1606915"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00395\u2010008\u20100743\u2010y"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/7018393"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2017.12.003"},{"issue":"4","key":"e_1_2_9_8_1","first-page":"180","article-title":"Mechanisms of Load Dependency of Myocardial Ischemia Reperfusion Injury","volume":"3","author":"Mozaffari M. 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