{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T07:42:25Z","timestamp":1792654945246,"version":"4.5.0"},"reference-count":70,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:00:00Z","timestamp":1792627200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:00:00Z","timestamp":1792627200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>As in vitro tissue models, including organoids, become thicker and more complex, diffusion\u2010limited three\u2010dimensional (3D) culture increasingly fails to sustain long\u2010term viability, nutrient supply, and spatial microenvironmental regulation. Existing vascularization, perfusion, and chip\u2010based strategies still struggle to integrate complex 3D architecture, durable perfusion, mechanical integrity, and controlled trans\u2010wall exchange in thick constructs. Here, a fibril\u2010reinforced, 3D\u2010printed solid\u2010walled zein vascular interface is developed to couple long\u2010term perfusion stability with wall\u2010mediated mass transport. Homologous zein fibrils enable direct printing of branched perfusable conduits with tunable porous walls, avoiding sacrificial hollow\u2010channel templating while improving deformation resistance and preserving permeability during 30 days of continuous perfusion. Wall architecture establishes two application\u2010specific transport regimes. In a regulated\u2010exchange regime, the interface supports nutrient delivery in 33 \u00d7 21 \u00d7 11\u00a0mm cell\u2010laden constructs, maintaining long\u2010term survival and enhancing CD31 expression within a continuously perfused peri\u2010conduit microenvironment. In a high\u2010permeability regime, enlarged porous walls generate spatial transport gradients, distance\u2010dependent heterogeneity, and enhanced permeability and retention (EPR)\u2010relevant trans\u2010wall nanocarrier transport with peri\u2010conduit accumulation, enabling evaluation of photoresponsive liposomal delivery and polyaniline\u2010mediated photothermal responses. This zein platform integrates printable biomaterial architecture, perfusion durability, and tunable wall\u2010mediated transport for thick tissue support and EPR\u2010relevant therapeutic evaluation in advanced in vitro model systems.<\/jats:p>","DOI":"10.1002\/adhm.71882","type":"journal-article","created":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T07:36:20Z","timestamp":1792654580000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["3D Printed Solid\u2010Walled Zein Vascular Network With Long\u2010Term Perfusion Stability and Trans\u2010Wall Transport"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-8478-7291","authenticated-orcid":false,"given":"Huicheng","family":"Fang","sequence":"first","affiliation":[{"name":"School of Biomedical Engineering Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya\u2010Qi","family":"Xue","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yubei","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yucheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering Shanghai Jiao Tong University  Shanghai China"},{"name":"Jiaxing Yaojiao Medical Device Co. Ltd.  Jiaxing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9282-2751","authenticated-orcid":false,"given":"Liping","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering Shanghai Jiao Tong University  Shanghai China"},{"name":"Wenzhou Institute University of Chinese Academy of Sciences  Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin\u2010Ye","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering Shanghai Jiao Tong University  Shanghai China"},{"name":"Jiaxing Yaojiao Medical Device Co. Ltd.  Jiaxing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,22]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/d41586\u2010025\u201003344\u20106"},{"key":"e_1_2_11_3_1","unstructured":"U. S. Food and D. Administration FDA announces plan to phase out animal testing requirement for monoclonal antibodies and other drugs 2025 https:\/\/www.fda.gov\/news\u2010events\/press\u2010announcements\/fda\u2010announces\u2010plan\u2010phase\u2010out\u2010animal\u2010testing\u2010requirement\u2010monoclonal\u2010antibodies\u2010and\u2010other\u2010drugs (accessed 28 March 2026)."},{"key":"e_1_2_11_4_1","unstructured":"National Institutes of Health NIH Funding Announcements to Align With NIH Initiative to Prioritize human\u2010based Research 2025 https:\/\/grants.nih.gov\/news\u2010events\/nih\u2010extramural\u2010nexus\u2010news\/2025\/07\/nih\u2010funding\u2010announcements\u2010to\u2010align\u2010with\u2010nih\u2010initiative\u2010to\u2010prioritize\u2010human\u2010based\u2010research (accessed 28 March 2026)."},{"key":"e_1_2_11_5_1","unstructured":"U. K. Government Replacing animals in science: a strategy to support the development validation and uptake of alternative methods 2025 https:\/\/www.gov.uk\/government\/publications\/replacing\u2010animals\u2010in\u2010science\u2010strategy\/replacing\u2010animals\u2010in\u2010science\u2010a\u2010strategy\u2010to\u2010support\u2010the\u2010development\u2010validation\u2010and\u2010uptake\u2010of\u2010alternative\u2010methods (accessed 28 March 2026)."},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2024.11.009"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cobme.2025.100586"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201045710\u20104"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010022\u201035619\u20101"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2023.09.019"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2024.1336910"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41378\u2010025\u201000956\u2010w"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003\u2010021\u201002285\u2010w"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201046928\u2010y"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s44222\u2010024\u201000207\u2010z"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1039\/D5LC01060H"},{"issue":"8","key":"e_1_2_11_17_1","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.ade7923","article-title":"High Cell Density and High\u2010Resolution 3D Bioprinting for Fabricating Vascularized Tissues","volume":"9","author":"You S.","year":"2023","journal-title":"Science advances"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crmeth.2024.100779"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mattod.2025.02.002"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talo.2025.100531"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bprint.2019.e00071"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2021.685507"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202509242"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/ctm2.70258"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40644\u2010024\u201000767\u20108"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioadv.2025.214636"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201052416\u20100"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2023.07.039"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2022.03.043"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010bioeng\u2010103023\u2010115236"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.07.064"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1039\/c000022a"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.2310\/7290.2012.00012"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/adtp.202500368"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0LC01186J"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2021.06.002"},{"key":"e_1_2_11_37_1","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.biomaterials.2016.07.038","article-title":"Direct 3D Bioprinting of Perfusable Vascular Constructs Using a Blend Bioink","volume":"106","author":"Jia W.","year":"2016","journal-title":"Biomaterials"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24010891"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13770\u2010025\u201000731\u2010y"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13346\u2010024\u201001580\u20103"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1039\/D4LC00968A"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1088\/1758\u20105090\/ae2f02"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2017.03.038"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2021.11.035"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioadv.2023.213473"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2006.02.019"},{"issue":"27","key":"e_1_2_11_47_1","doi-asserted-by":"crossref","first-page":"3952","DOI":"10.1016\/j.biomaterials.2007.05.017","article-title":"In Vivo Biocompatibility and Mechanical Properties of Porous Zein Scaffolds","volume":"28","author":"Wang H. J.","year":"2007","journal-title":"Biomaterials"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2017.01.035"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1039\/D5MA00663E"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.3c01978"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.4c01824"},{"key":"e_1_2_11_52_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodhyd.2024.110926","article-title":"The Formation Mechanism of Zein Gel Induced by Amyloid\u2010Like Fibrils","volume":"162","author":"Fang H.","year":"2025","journal-title":"Food Hydrocolloids"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1088\/0957\u20104484\/25\/27\/275103"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.2c16480"},{"key":"e_1_2_11_55_1","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/j.bioactmat.2022.11.009","article-title":"Water\u2010Responsive 4D Printing Based on Self\u2010Assembly of Hydrophobic Protein \u201cZein\u201d for the Control of Degradation Rate and Drug Release","volume":"23","author":"Zhang Y.","year":"2023","journal-title":"Bioactive Materials"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.polymdegradstab.2020.109303"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep39202"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1521342113"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.202500240"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11517\u2010008\u20100330\u20102"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078\u20100432.CCR\u201005\u20101664"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102407\u201010\u2010255"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.1021\/nl080496z"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.70161"},{"key":"e_1_2_11_65_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2014.00052"},{"key":"e_1_2_11_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bea.2022.100059"},{"key":"e_1_2_11_67_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010022\u201024423\u2010y"},{"key":"e_1_2_11_68_1","doi-asserted-by":"publisher","DOI":"10.3390\/gels10050316"},{"key":"e_1_2_11_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2020.11.006"},{"key":"e_1_2_11_70_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010physiol\u2010021909\u2010135833"},{"key":"e_1_2_11_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142\u20109612(98)00107\u20100"}],"container-title":["Advanced Healthcare Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.71882","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/adhm.71882","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.71882","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T07:36:23Z","timestamp":1792654583000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adhm.71882"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,22]]},"references-count":70,"alternative-id":["10.1002\/adhm.71882"],"URL":"https:\/\/doi.org\/10.1002\/adhm.71882","archive":["Portico"],"relation":{},"ISSN":["2192-2640","2192-2659"],"issn-type":[{"value":"2192-2640","type":"print"},{"value":"2192-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,22]]},"assertion":[{"value":"2026-04-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71882"}}