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Gene transfection to make NSCs overexpress brain\u2010derived neurotrophic factor (BDNF) can enhance their therapeutic efficacy; however, viral vectors must be used because current nonviral vectors are unable to efficiently transfect NSCs. The first polymeric vector, ROS\u2010responsive charge\u2010reversal poly[(2\u2010acryloyl)ethyl(<jats:italic>p<\/jats:italic>\u2010boronic acid benzyl)diethylammonium bromide] (B\u2010PDEA), is shown here, that mediates efficient gene transfection of NSCs and greatly enhances their therapeutics in ischemic stroke treatment. The cationic B\u2010PDEA\/DNA polyplexes can effectively transfect NSCs; in the cytosol, the B\u2010PDEA is oxidized by intracellular ROS into negatively charged polyacrylic acid, quickly releasing the BDNF plasmids for efficient transcription and secreting a high level of BDNF. After i.v. injection in ischemic stroke mice, the transfected NSCs (BDNF\u2010NSCs) can home to ischemic regions as efficiently as the pristine NSCs but more efficiently produce BDNF, leading to significantly augmented BDNF levels, which in turn enhances the mouse survival rate to 60%, from 0% (nontreated mice) or \u224820% (NSC\u2010treated mice), and enables more rapid and superior functional reconstruction.<\/jats:p>","DOI":"10.1002\/adma.201807591","type":"journal-article","created":{"date-parts":[[2019,1,11]],"date-time":"2019-01-11T09:42:29Z","timestamp":1547199749000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":140,"title":["Neural Stem Cells Transfected with Reactive Oxygen Species\u2013Responsive Polyplexes for Effective Treatment of Ischemic Stroke"],"prefix":"10.1002","volume":"31","author":[{"given":"Xin\u2010Chi","family":"Jiang","sequence":"first","affiliation":[{"name":"Institute of Pharmaceutics College of Pharmaceutical Sciences Zhejiang University  Hangzhou Zhejiang 310058 P. 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