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Poly(ethylene glycol) (PEG) is usually applied to modify cationic polymers, so as to reduce protein and cell adsorption and then to improve siRNA transfection efficiency. However, the polymers\u2019 modification with PEG mostly consumes the free amino of the polymers, which can, in turn, reduce the charge density and limit their siRNA transfection efficacy. Here, a new PEG modification strategy that need not consume the surface aminos of polymers is proposed. Catechol\u2013PEG polymers are coated on the surface of phenylboronic acid (PBA)\u2010modified Generation 5 (G5) poly(amidoamine) dendrimers (G5PBA) via reversible boronate esters to establish PEG\u2010modified dendrimer\/siRNA nanoassemblies for efficient siRNA delivery. The PEG\/G5PBA\/siRNA nanoassemblies have positive charge and show excellent gene silencing efficacy in the absence of serum in vitro. More importantly, the PEG\/G5PBA\/siRNA nanoassemblies also exhibit excellent serum resistance and gene silencing efficacy in serum\u2010containing medium. Furthermore, the effective antiserum and gene silencing efficacy elicited by these nanoassemblies lead to excellent antitumor effects in vivo. This proposed strategy constitutes an important approach to reach an excellent gene silencing efficacy in the presence of serum.<\/jats:p>","DOI":"10.1002\/adma.202003523","type":"journal-article","created":{"date-parts":[[2020,12,24]],"date-time":"2020-12-24T08:11:23Z","timestamp":1608797483000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Nanoassemblies with Effective Serum Tolerance Capability Achieving Robust Gene Silencing Efficacy for Breast Cancer Gene Therapy"],"prefix":"10.1002","volume":"33","author":[{"given":"Hongmei","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oncogenes and Related Genes Shanghai Cancer Institute Renji Hospital Shanghai Jiao Tong University School of Medicine  Shanghai 200032 China"},{"name":"Central Laboratory Renji Hospital School of Medicine Shanghai Jiao Tong University  Shanghai 200127 China"},{"name":"Micro\u2010Nano Research and Diagnosis Center Renji Hospital School of Medicine Shanghai Jiao Tong University  Shanghai 2000127 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chongyi","family":"Liu","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Regulatory Biology School of Life Sciences East China Normal University  Shanghai 200241 P. 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