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However, clinical translation of CRISPR technology is limited by the lack of safe and efficient delivery systems. Here, facilely fabricated pH\u2010responsive polymer nanoparticles capable of safely and efficiently delivering Cas9 ribonucleoprotein alone (termed NHEJ\u2010NP, diameter = 29.4\u00a0nm), or together with donor DNA (termed HDR\u2010NP, diameter = 33.3\u00a0nm) are reported. Moreover, intravenously, intratracheally, and intramuscularly injected NHEJ\u2010NP induces efficient gene editing in mouse liver, lung, and skeletal muscle, respectively. Intramuscularly injected HDR\u2010NP also leads to muscle strength recovery in a Duchenne muscular dystrophy mouse model. NHEJ\u2010NP and HDR\u2010NP possess many desirable properties including high payload loading content, small and uniform sizes, high editing efficiency, good biocompatibility, low immunogenicity, and ease of production, storage, and transport, making them great interest for various genome editing applications with clinical potentials.<\/jats:p>","DOI":"10.1002\/adma.202110618","type":"journal-article","created":{"date-parts":[[2022,2,4]],"date-time":"2022-02-04T07:59:08Z","timestamp":1643961548000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":61,"title":["pH\u2010Responsive Polymer Nanoparticles for Efficient Delivery of Cas9 Ribonucleoprotein With or Without Donor DNA"],"prefix":"10.1002","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0249-0002","authenticated-orcid":false,"given":"Ruosen","family":"Xie","sequence":"first","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences University 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