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A lack of automation and precision limits LIFT applications to simple proof\u2010of\u2010concept syntheses of fewer than 100 compounds. Here, an automated synthesis instrument is reported that combines laser transfer and robotics for parallel synthesis in a microarray format with up to 10\u2009000 individual reactions cm<jats:sup>\u2212<\/jats:sup><jats:sup>2<\/jats:sup>. An optimized pipeline for amide bond formation is the basis for preparing complex peptide microarrays with thousands of different sequences in high yield with high reproducibility. The resulting peptide arrays are of higher quality than commercial peptide arrays. More than 4800 15\u2010residue peptides resembling the entire Ebola virus proteome on a microarray are synthesized to study the antibody response of an Ebola virus infection survivor. Known and unknown epitopes that serve now as a basis for Ebola diagnostic development are identified. The versatility and precision of the synthesizer is demonstrated by in situ synthesis of fluorescent molecules via Schiff base reaction and multi\u2010step patterning of precisely definable amounts of fluorophores. This automated laser transfer synthesis approach opens new avenues for high\u2010throughput chemical synthesis and biological screening.<\/jats:p>","DOI":"10.1002\/adma.202200359","type":"journal-article","created":{"date-parts":[[2022,4,16]],"date-time":"2022-04-16T00:36:59Z","timestamp":1650069419000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Automated Laser\u2010Transfer Synthesis of High\u2010Density Microarrays for Infectious Disease Screening"],"prefix":"10.1002","volume":"34","author":[{"given":"Grigori","family":"Paris","sequence":"first","affiliation":[{"name":"Department of Biomolecular Systems Max Planck Institute of Colloids and Interfaces  Am Muehlenberg 1 14476 Potsdam Germany"},{"name":"Department of System Dynamics and Friction Physics Institute of Mechanics Technical University of Berlin  Str. des 17. 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