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In this study, by combining the design concepts of needle\u2010type microelectrodes and the fluorescence imaging method, a new technique is developed for detecting biomarkers in vivo, named as \u201coptically controlled virtual microsensor\u201d (OCViM). OCViM is established by the organic integration of a specially shaped laser beam and fluorescent nanoprobe, which serve as the virtual handle and sensor tip, respectively. The laser beam can trap and manipulate the nanoprobe in a programmable manner, and meanwhile excite it to generate fluorescence emission for biosensing. On this basis, fully active control of the nanoprobe is achieved noninvasively in vivo, and multipoint detection can be realized at sub\u2010micrometer resolution by shifting a nanoprobe among multiple positions. By using OCViM, the overexpression and heterogenous distribution of biomarkers in the thrombus is studied in living zebrafish, which is further utilized for the evaluation of antithrombotic drugs. OCViM may provide a powerful tool for the mechanism study of thrombus progression and the evaluation of antithrombotic drugs.<\/jats:p>","DOI":"10.1002\/adma.202205760","type":"journal-article","created":{"date-parts":[[2022,9,8]],"date-time":"2022-09-08T15:20:03Z","timestamp":1662650403000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["An Optically Controlled Virtual Microsensor for Biomarker Detection In Vivo"],"prefix":"10.1002","volume":"34","author":[{"given":"Tiange","family":"Zhang","sequence":"first","affiliation":[{"name":"Institute of Nanophotonics Jinan University  Guangzhou 511443 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Nanophotonics Jinan University  Guangzhou 511443 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haifeng","family":"Qin","sequence":"additional","affiliation":[{"name":"Institute of Nanophotonics Jinan University  Guangzhou 511443 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huaying","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Nanophotonics Jinan University  Guangzhou 511443 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoshuai","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Nanophotonics Jinan University  Guangzhou 511443 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baojun","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Nanophotonics Jinan University  Guangzhou 511443 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1076-8959","authenticated-orcid":false,"given":"Xianchuang","family":"Zheng","sequence":"additional","affiliation":[{"name":"Institute of Nanophotonics Jinan University  Guangzhou 511443 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,10,3]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.9b00437"},{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.6b10646"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/es070969o"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2017.04.043"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2013.5378"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-19-0530"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.8b10176"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201601214"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.8b02881"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202003451"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202007673"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201804827"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/lsa.2017.39"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.8b01844"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2017.06.025"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2021.213870"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1134\/S0030400X15040190"},{"key":"e_1_2_9_10_1","first-page":"035003","volume":"24","author":"Liu S.","year":"2019","journal-title":"J. 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