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Finding an appropriate material host for de novo designed protein switches without altering their thermodynamics while preserving their intrinsic stability over time would enable the development of a variety of sensing formats to monitor exposure to pathogens, toxins, and for disease diagnosis. Here, a de novo protein\u2010biopolymer hybrid that maintains the detection capabilities induced by the conformational change of the incorporated proteins\u00a0in response to analytes of interest is generated in multiple, shelf\u2010stable material formats without the need of refrigerated storage conditions. A set of functional demonstrator devices including personal protective equipment such as masks and laboratory gloves, free\u2010standing films, air quality monitors, and wearable devices is presented to illustrate the versatility of the approach. Such formats are designed to be responsive to human epidermal growth factor receptor (HER2), anti\u2010hepatitis B (HBV) antibodies, Botulinum neurotoxin B (BoNT\/B), and severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102). This combination of form and function offers wide opportunities for ubiquitous sensing in multiple environments by enabling a large class of bio\u2010responsive interfaces of broad utility.<\/jats:p>","DOI":"10.1002\/adma.202208556","type":"journal-article","created":{"date-parts":[[2022,12,9]],"date-time":"2022-12-09T16:19:00Z","timestamp":1670602740000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Reshaping de Novo Protein Switches into Bioresponsive Materials for Biomarker, Toxin, and Viral Detection"],"prefix":"10.1002","volume":"35","author":[{"given":"Luciana","family":"d'Amone","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering Tufts University  Medford MA 02155 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giusy","family":"Matzeu","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Tufts University  Medford MA 02155 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