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The pH\u2010dependent tunable polarity of the LAF is successfully implemented by inserting a central tertiary amine, which disrupts the hydrophobic character once protonated, resulting in pH\u2010dependent structural and physical changes. This \u201cmolecular chameleon character\u201d turns out to be advantageous for dynamic nucleic acid delivery via lipopolyplexes. By screening different topologies (blocks, bundles, T\u2010shapes, U\u2010shapes), LAF types, and LAF\/aminoethylene ratios, highly potent pDNA, mRNA, and siRNA carriers are identified, which are up to several 100\u2010fold more efficient than previous carrier generations and characterized by very fast transfection kinetics. mRNA lipopolyplexes maintain high transfection activity in cell culture even in the presence of \u226590% serum at an ultra\u2010low mRNA dose of 3 picogram (\u22482 nanoparticles\/cell), and thus are comparable in potency to viral nanoparticles. 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