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In this study, specially designed lacunar hyaluronic acid microcarriers (LHAMCs) with mechanotransductive conditions that rapidly form stable hyaluronic acid (HA) <jats:italic>N<\/jats:italic>\u2010hydroxy succinimide ester (NHS\u2010ester) are reported. Specifically, carboxyl\u2010functionalized HA is linked to collagen type I via amide\u2010crosslinking, and gas foaming produced by ammonium bicarbonate forms concave surface of the microcarriers. The temporal 3D culture of chondrocytes on LHAMCs uniquely remodels the extracellular matrix to induce hyaline cartilaginous microtissue regeneration and prevents an anaerobic\u2010to\u2010aerobic metabolism transition in response to the geometric constraints. Furthermore, by inhibiting the canonical Wnt pathway, LHAMCs prevent <jats:italic>\u03b2<\/jats:italic>\u2010catenin translocation to the nucleus, repressing chondrocyte dedifferentiation. 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