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However, SPNs are susceptible to aggregation and protein fouling in physiological conditions, which can be detrimental for in vivo applications. Here, a method for achieving colloidally stable and low\u2010fouling SPNs is described by grafting poly(ethylene glycol) (PEG) onto the backbone of the fluorescent semiconducting polymer, poly(9,9\u2032\u2010dioctylfluorene\u20105\u2010fluoro\u20102,1,3\u2010benzothiadiazole), in a simple one\u2010step substitution reaction, postpolymerization. Further, by utilizing azide\u2010functionalized PEG, anti\u2010human epidermal growth factor receptor 2 (HER2)\u00a0antibodies, antibody fragments, or affibodies are site\u2010specifically \u201cclicked\u201d onto the SPN surface, which allows the functionalized SPNs to specifically target HER2\u2010positive cancer cells. In vivo, the PEGylated SPNs are found to have excellent circulation efficiencies in zebrafish embryos for up to seven days postinjection. 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The covalent PEGylated SPN system described herein shows great potential for cancer theranostics.<\/jats:p>","DOI":"10.1002\/adma.202300413","type":"journal-article","created":{"date-parts":[[2023,3,11]],"date-time":"2023-03-11T17:03:55Z","timestamp":1678554235000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Modular Synthesis of Semiconducting Graft Copolymers to Achieve \u201cClickable\u201d Fluorescent Nanoparticles with Long Circulation and Specific Cancer Targeting"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6203-8456","authenticated-orcid":false,"given":"Adam","family":"Creamer","sequence":"first","affiliation":[{"name":"Department of Materials Department of Bioengineering Institute of Biomedical Engineering Imperial College London  London SW7 2AZ UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5797-8884","authenticated-orcid":false,"given":"Alessandra Lo","family":"Fiego","sequence":"additional","affiliation":[{"name":"Department of Materials Department of Bioengineering Institute of Biomedical Engineering Imperial College London  London SW7 2AZ UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4846-8086","authenticated-orcid":false,"given":"Alice","family":"Agliano","sequence":"additional","affiliation":[{"name":"Department of Materials Department of Bioengineering Institute of Biomedical Engineering Imperial College London  London SW7 2AZ UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2196-3706","authenticated-orcid":false,"given":"Lino","family":"Prados\u2010Martin","sequence":"additional","affiliation":[{"name":"Department of Materials Department of Bioengineering Institute of Biomedical Engineering Imperial College London  London SW7 2AZ UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e5kon","family":"H\u00f8gset","sequence":"additional","affiliation":[{"name":"Department of Materials Department of Bioengineering Institute of Biomedical Engineering Imperial College London  London SW7 2AZ UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4868-9364","authenticated-orcid":false,"given":"Adrian","family":"Najer","sequence":"additional","affiliation":[{"name":"Department of Materials Department of Bioengineering Institute of Biomedical Engineering Imperial College London  London SW7 2AZ UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8827-9170","authenticated-orcid":false,"given":"Daniel A.","family":"Richards","sequence":"additional","affiliation":[{"name":"Department of Materials Department of Bioengineering Institute of Biomedical Engineering Imperial College London  London SW7 2AZ UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6272-515X","authenticated-orcid":false,"given":"Jonathan P.","family":"Wojciechowski","sequence":"additional","affiliation":[{"name":"Department of Materials Department of Bioengineering Institute of Biomedical Engineering Imperial College London  London SW7 2AZ UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0161-1386","authenticated-orcid":false,"given":"James E. 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