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However, conventional delivery methods fall short in addressing the heightened inflammatory response orchestrated by macrophages during the acute stages of tendon injury. In this investigation, macrophage\u2010targeting cationic polymers are systematically screened to facilitate the entry of Cas13 ribonucleic\u2010protein complex (Cas13 RNP) into macrophages. Notably, SPP1 (OPN encoding)\u2010producing macrophages are recognized as a profibrotic subtype that emerges during the inflammatory stage. By employing ROS\u2010responsive release mechanisms tailored for macrophage\u2010targeted Cas13 RNP editing systems, the overactivation of SPP1 is curbed in the face of an acute immune microenvironment. Upon encapsulating this composite membrane around the tendon injury site, the macrophage\u2010targeted Cas13 RNP effectively curtails the emergence of injury\u2010induced SPP1\u2010producing macrophages in the acute phase, leading to diminished fibroblast activation and mitigated peritendinous adhesion. Consequently, this study furnishes a swift RNA editing strategy for macrophages in the inflammatory phase triggered by ROS in tendon injury, along with a pioneering macrophage\u2010targeted carrier proficient in delivering Cas13 into macrophages efficiently.<\/jats:p>","DOI":"10.1002\/adma.202311964","type":"journal-article","created":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T19:34:25Z","timestamp":1706816065000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Targeted Macrophage CRISPR\u2010Cas13 mRNA Editing in Immunotherapy for Tendon Injury"],"prefix":"10.1002","volume":"36","author":[{"given":"Shuo","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Orthopaedics Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai 200233 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