{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T16:54:46Z","timestamp":1779209686798,"version":"3.51.4"},"reference-count":82,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2024,4,4]],"date-time":"2024-04-04T00:00:00Z","timestamp":1712188800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["ZIABC011313"],"award-info":[{"award-number":["ZIABC011313"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["ZIABC011657"],"award-info":[{"award-number":["ZIABC011657"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Materials"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Broad size distributions and poor long\u2010term colloidal stability of microRNA\u2010carrying nanoparticles, especially those formed by polyelectrolyte complexation, represent major hurdles in realizing their clinical translation. Herein, peptide design is used alongside optimized flash nanocomplexation (FNC) to produce uniform peptide\u2010based miRNA particles of exceptional stability that display anticancer activity against mesothelioma in vitro and in vivo. Modulating the content and display of lysine\u2010based charge from small intrinsically disordered peptides used to complex miRNA proves essential in achieving stable colloids. FNC facilitates kinetic isolation of the mechanistic steps involved in particle formation to allow the preparation of particles of discrete size in a highly reproducible, scalable, and continuous manner, facilitating pre\u2010clinical studies. To the best of the authors knowledge, this work represents the first example of employing FNC to prepare polyelectrolyte complexes of miRNA and peptide. Encapsulation of these particles into an injectable hydrogel matrix allows for their localized in vivo delivery by syringe. A one\u2010time injection of a gel containing particles composed of miRNA\u2010215\u20105p and the peptide PKM1 limits tumor progression in a xenograft model of mesothelioma.<\/jats:p>","DOI":"10.1002\/adma.202314367","type":"journal-article","created":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T02:44:47Z","timestamp":1711507487000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Kinetically Controlled Polyelectrolyte Complex Assembly of microRNA\u2010Peptide Nanoparticles toward Treating Mesothelioma"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0760-1928","authenticated-orcid":false,"given":"Caleb F.","family":"Anderson","sequence":"first","affiliation":[{"name":"Chemical Biology Laboratory National Cancer Institute National Institutes of Health  Frederick MD 21701 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anand","family":"Singh","sequence":"additional","affiliation":[{"name":"Thoracic Surgery Branch National Cancer Institute National Institutes of Health  Bethesda MD 20892 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tyler","family":"Stephens","sequence":"additional","affiliation":[{"name":"Vaccine Research Center Electron Microscopy Unit Cancer Research Technology Program Frederick National Laboratory for Cancer Research  Frederick MD 20701 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuong D.","family":"Hoang","sequence":"additional","affiliation":[{"name":"Thoracic Surgery Branch National Cancer Institute National Institutes of Health  Bethesda MD 20892 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4403-7900","authenticated-orcid":false,"given":"Joel P.","family":"Schneider","sequence":"additional","affiliation":[{"name":"Chemical Biology Laboratory National Cancer Institute National Institutes of Health  Frederick MD 21701 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,4,4]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-021-00439-4"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-022-01294-2"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41578-021-00358-0"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2009.04.001"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2022.01.008"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matt.2020.09.020"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemmater.1c01832"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573-021-00283-5"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2021.114083"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41565-021-00961-w"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2019.05.020"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nantod.2016.04.006"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.5b06890"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2012.168"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.1c01421"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202106388"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.31854"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2015.12.032"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.0c00997"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573-020-0075-7"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1322937111"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2014.84"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2010.10.028"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5277"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1810002115"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201805097"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscentsci.9b00843"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201601412"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja506194z"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.12.022"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-26695-w"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja804561g"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1039\/C1CS15258K"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1502281112"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abk2855"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mattod.2020.08.019"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2011.04.005"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/nn501371n"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201601425"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/aic.690490905"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1071\/CH03115"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevLett.91.118302"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcis.2010.07.027"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1063\/5.0002125"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.9b03334"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.1c01353"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.3c01117"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcis.2022.12.028"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtm.2024.101194"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1021\/ma0711689"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp301069t"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpcb.1c01258"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp501234p"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.6b01247"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.7b03567"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cocis.2021.101424"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp0277107"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsmacrolett.0c00543"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201103348"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja027993g"},{"key":"e_1_2_7_61_1","first-page":"3","volume-title":"Advances in Nanomedicine for the Delivery of Therapeutic Nucleic Acids","author":"Kumar A.","year":"2017"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.8b06309"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1021\/ma025737i"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.macromol.1c00743"},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.2c00338"},{"key":"e_1_2_7_66_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.1c01148"},{"key":"e_1_2_7_67_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.8b03132"},{"key":"e_1_2_7_68_1","doi-asserted-by":"publisher","DOI":"10.3390\/polym11010083"},{"key":"e_1_2_7_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2021.01.001"},{"key":"e_1_2_7_70_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201703596"},{"key":"e_1_2_7_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2019.110497"},{"key":"e_1_2_7_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-3659(02)00131-1"},{"key":"e_1_2_7_73_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja710295t"},{"key":"e_1_2_7_74_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm900113z"},{"key":"e_1_2_7_75_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2006.09.017"},{"key":"e_1_2_7_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2020.03.001"},{"key":"e_1_2_7_77_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2022.02.001"},{"key":"e_1_2_7_78_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpb.2021.05.024"},{"key":"e_1_2_7_79_1","doi-asserted-by":"publisher","DOI":"10.1039\/D3TB00308F"},{"key":"e_1_2_7_80_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2018.09.035"},{"key":"e_1_2_7_81_1","doi-asserted-by":"publisher","DOI":"10.1021\/js980001d"},{"key":"e_1_2_7_82_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.21786"}],"container-title":["Advanced Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adma.202314367","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T14:12:05Z","timestamp":1759759925000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adma.202314367"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,4]]},"references-count":82,"journal-issue":{"issue":"24","published-print":{"date-parts":[[2024,6]]}},"alternative-id":["10.1002\/adma.202314367"],"URL":"https:\/\/doi.org\/10.1002\/adma.202314367","archive":["Portico"],"relation":{},"ISSN":["0935-9648","1521-4095"],"issn-type":[{"value":"0935-9648","type":"print"},{"value":"1521-4095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,4]]},"assertion":[{"value":"2023-12-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2314367"}}