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However, some microbes have developed strategies to evade NET attacks, leading to impaired immune defenses and persistent infections. In this study, an engineered neutrophil strategy for enhancing the antibacterial activity of NETs is developed. A nucleus\u2010targeting photosensitizer (NCP) with strong reactive oxygen species production and a strong DNA\u2010binding capacity is synthesized. NCP\u2010loaded neutrophils are subsequently constructed via direct incubation of NCP with neutrophils, and the NCP is closely inserted into the nucleus DNA. Upon activation by bacteria\u2010related toxins, NCP\u2010coupled NETs can be released rapidly, actively trapping bacteria and providing a high local concentration of NCP around them. Both in vitro and in vivo results revealed that NCP\u2010coupled NETs can effectively eradicate various multidrug\u2010resistant bacteria and biofilms through photodynamic therapy, overcome bacterial immune evasion, and promote tissue recovery from severe wound infections. This design can significantly strengthen NET function, providing a non\u2010antibiotic alternative platform for treating bacterial infectious diseases.<\/jats:p>","DOI":"10.1002\/adma.202400304","type":"journal-article","created":{"date-parts":[[2024,11,12]],"date-time":"2024-11-12T03:15:40Z","timestamp":1731381340000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Nucleus\u2010Targeting Photosensitizers Enhance Neutrophil Extracellular Traps for Efficient Eradication of Multidrug\u2010Resistant Bacterial Infections"],"prefix":"10.1002","volume":"36","author":[{"given":"Peng","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Burns Wound Repair &amp; Reconstruction Institute of Precision Medicine The First Affiliated Hospital of Sun Yat\u2010Sen University Sun Yat\u2010Sen University  Guangzhou 510080 China"},{"name":"Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering Biomaterials Research Center School of Biomedical Engineering Southern Medical University  Guangzhou 510515 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingqing","family":"Bai","sequence":"additional","affiliation":[{"name":"Department of Burns Wound Repair &amp; 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