{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T16:06:34Z","timestamp":1793117194710,"version":"4.5.0"},"reference-count":69,"publisher":"Wiley","issue":"32","license":[{"start":{"date-parts":[[2024,6,10]],"date-time":"2024-06-10T00:00:00Z","timestamp":1717977600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100005156","name":"Alexander von Humboldt-Stiftung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005156","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Materials"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Mucus is a dynamic biological hydrogel, composed primarily of the glycoprotein mucin, exhibits unique biophysical properties and forms a barrier protecting cells against a broad\u2010spectrum of viruses. Here, this work develops a polyglycerol sulfate\u2010based dendronized mucin\u2010inspired copolymer (\n                    <jats:bold>MICP\u20101<\/jats:bold>\n                    ) with \u224810% repeating units of activated disulfide as cross\u2010linking sites. Cryo\u2010electron microscopy (Cryo\u2010EM)\u00a0analysis of\n                    <jats:bold>MICP\u20101<\/jats:bold>\n                    reveals an elongated single\u2010chain fiber morphology.\n                    <jats:bold>MICP\u20101<\/jats:bold>\n                    shows potential inhibitory activity against many viruses such as herpes simplex virus 1 (HSV\u20101) and SARS\u2010CoV\u20102 (including variants such as Delta and Omicron).\n                    <jats:bold>MICP\u20101<\/jats:bold>\n                    produces hydrogels with viscoelastic properties similar to healthy human sputum and with tuneable microstructures using linear and branched polyethylene glycol\u2010thiol (PEG\u2010thiol) as cross\u2010linkers. Single particle tracking microrheology, electron paramagnetic resonance (EPR) and cryo\u2010scanning electron microscopy (Cryo\u2010SEM) are used to characterize the network structures. The synthesized hydrogels exhibit self\u2010healing properties, along with viscoelastic properties that are tuneable through reduction. A transwell assay is used to investigate the hydrogel's protective properties against viral infection against HSV\u20101. Live\u2010cell microscopy confirms that these hydrogels can protect underlying cells from infection by trapping the virus, due to both network morphology and anionic multivalent effects. Overall, this novel mucin\u2010inspired copolymer generates mucus\u2010mimetic hydrogels on a multi\u2010gram scale. These hydrogels can be used as models for disulfide\u2010rich airway mucus research, and as biomaterials.\n                  <\/jats:p>","DOI":"10.1002\/adma.202401745","type":"journal-article","created":{"date-parts":[[2024,5,30]],"date-time":"2024-05-30T15:20:20Z","timestamp":1717082420000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Mucus\u2010Inspired Self\u2010Healing Hydrogels: A Protective Barrier for Cells against Viral Infection"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8453-6966","authenticated-orcid":false,"given":"Raju","family":"Bej","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Chemie und Biochemie Freie Universit\u00e4t Berlin Takustrasse 3  14195 Berlin Germany"},{"name":"Department of Biological Engineering Massachusetts Institute of Technology  Cambridge MA 02139 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