{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T22:33:31Z","timestamp":1792794811980,"version":"4.5.0"},"reference-count":56,"publisher":"Wiley","issue":"33","license":[{"start":{"date-parts":[[2024,6,23]],"date-time":"2024-06-23T00:00:00Z","timestamp":1719100800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2020YFA0908200"],"award-info":[{"award-number":["2020YFA0908200"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFC2701001"],"award-info":[{"award-number":["2021YFC2701001"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81930051"],"award-info":[{"award-number":["81930051"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82202455"],"award-info":[{"award-number":["82202455"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["52103174"],"award-info":[{"award-number":["52103174"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2022M712131"],"award-info":[{"award-number":["2022M712131"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2022M720787"],"award-info":[{"award-number":["2022M720787"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["22YF1441800"],"award-info":[{"award-number":["22YF1441800"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Materials"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Excessive cell\u2010free DNA (cfDNA) can induce chronic inflammation by activating intracellular nucleic acid sensors. Intervention in cfDNA\u2010mediated \u201cpro\u2010inflammatory signaling transduction\u201d could be a potential alleviating strategy for chronic inflammation, such as in diabetic wounds. However, effectively and specifically downgrading cfDNA concentration in the pathological microenvironment remains a challenge. Therefore, this work prepares free\u2010standing polydopamine nanosheets through DNA\u2010guided assembly and loaded them into microfluidic hydrogel microspheres. The \u03c0\u2500\u03c0 stacking\/hydrogen bonding interactions between polydopamine nanosheets and the \u03c0\u2010rich bases of cfDNA, along with the cage\u2010like spatial confinement created by the hydrogel polymer network, achieved cfDNA capture and storage, respectively. Catechol in polydopamine nanosheets can also assist in reducing reactive oxygen species (ROS) levels. Efficient cfDNA binding independent of serum proteins, specific interdiction of abnormal activation of cfDNA\u2010associated toll\u2010like receptor 9, as well as down\u2010regulation of inflammatory cytokines and ROS levels are shown in this system. The chronic inflammation alleviating and the pro\u2010healing effects on the mice model with diabetic wounds are also investigated. This work presents a new strategy for capturing and storing cfDNA to intervene in cell signaling transduction. It also offers new insights into the regulatory mechanisms between inflammatory mediators and biomaterials in inflammation\u2010related diseases.<\/jats:p>","DOI":"10.1002\/adma.202403557","type":"journal-article","created":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T05:11:34Z","timestamp":1718601094000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Capture and Storage of Cell\u2010Free DNA via Bio\u2010Informational Hydrogel Microspheres"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-9066-0479","authenticated-orcid":false,"given":"Tao","family":"Ding","sequence":"first","affiliation":[{"name":"Department of Orthopaedics Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases Shanghai Institute of Traumatology and Orthopaedics Ruijin Hospital Shanghai Jiao Tong University School of Medicine  197 Ruijin 2nd Road Shanghai 200025 P. R. China"},{"name":"Jiaxing Key Laboratory of Basic Research and Clinical Translation on Orthopedic Biomaterials Department of Orthopaedics the Second Affiliated Hospital of Jiaxing University  Jiaxing 314000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongqiang","family":"Xiao","sequence":"additional","affiliation":[{"name":"ENT Institute Department of Facial Plastic and Reconstructive Surgery Eye &amp; ENT Hospital Fudan University  Shanghai 200031 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qimanguli","family":"Saiding","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases Shanghai Institute of Traumatology and Orthopaedics Ruijin Hospital Shanghai Jiao Tong University School of Medicine  197 Ruijin 2nd Road Shanghai 200025 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxiao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases Shanghai Institute of Traumatology and Orthopaedics Ruijin Hospital Shanghai Jiao Tong University School of Medicine  197 Ruijin 2nd Road Shanghai 200025 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Chen","sequence":"additional","affiliation":[{"name":"Jiaxing Key Laboratory of Basic Research and Clinical Translation on Orthopedic Biomaterials Department of Orthopaedics the Second Affiliated Hospital of Jiaxing University  Jiaxing 314000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"ENT Institute Department of Facial Plastic and Reconstructive Surgery Eye &amp; ENT Hospital Fudan University  Shanghai 200031 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Ma","sequence":"additional","affiliation":[{"name":"ENT Institute Department of Facial Plastic and Reconstructive Surgery Eye &amp; ENT Hospital Fudan University  Shanghai 200031 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6938-9582","authenticated-orcid":false,"given":"Wenguo","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases Shanghai Institute of Traumatology and Orthopaedics Ruijin Hospital Shanghai Jiao Tong University School of Medicine  197 Ruijin 2nd Road Shanghai 200025 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,6,23]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2021.03.007"},{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41578-022-00426-z"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2021.04.005"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2020.01.012"},{"key":"e_1_2_8_2_3","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.11.050"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2022.03.016"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.abn1721"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-022-00377-3"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.2c00526"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abj2372"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aay7148"},{"key":"e_1_2_8_6_3","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.0c04759"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nantod.2021.101358"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/171737a0"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/546687a"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-06603-5"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202000391"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-33492-6"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1039\/D3NR03231K"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202213465"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202111698"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2023.122366"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2021.113353"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cis.2022.102689"},{"key":"e_1_2_8_15_3","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202205713"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8NH00351C"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202103339"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssuschemeng.8b03429"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.memsci.2018.02.009"},{"key":"e_1_2_8_18_3","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202006270"},{"key":"e_1_2_8_18_4","doi-asserted-by":"publisher","DOI":"10.1126\/science.1147241"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.3c00302"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.1c02483"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-18654-8"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2022.03.028"},{"key":"e_1_2_8_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202300604"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/nn100816s"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.0c07028"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202213151"},{"key":"e_1_2_8_25_2","doi-asserted-by":"publisher","DOI":"10.1039\/C8CC05989F"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/admi.202000632"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2019.02.032"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.1c11301"},{"key":"e_1_2_8_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202007993"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmat4294"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202108325"},{"key":"e_1_2_8_30_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abn7006"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-42542-6"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI163541"},{"key":"e_1_2_8_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-023-02774-x"},{"key":"e_1_2_8_32_3","doi-asserted-by":"publisher","DOI":"10.1038\/s43018-022-00415-9"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034515589289"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-987X(199709)18:12<1463::AID-JCC4>3.0.CO;2-H"},{"key":"e_1_2_8_34_2","doi-asserted-by":"publisher","DOI":"10.1063\/1.470117"},{"key":"e_1_2_8_34_3","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jctc.5b00716"},{"key":"e_1_2_8_34_4","doi-asserted-by":"publisher","DOI":"10.1021\/ct700301q"}],"container-title":["Advanced Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adma.202403557","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T12:59:44Z","timestamp":1759755584000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adma.202403557"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,23]]},"references-count":56,"journal-issue":{"issue":"33","published-print":{"date-parts":[[2024,8]]}},"alternative-id":["10.1002\/adma.202403557"],"URL":"https:\/\/doi.org\/10.1002\/adma.202403557","archive":["Portico"],"relation":{},"ISSN":["0935-9648","1521-4095"],"issn-type":[{"value":"0935-9648","type":"print"},{"value":"1521-4095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,23]]},"assertion":[{"value":"2024-03-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2403557"}}