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To meet the growing demand for materials used for high throughput mechanotransduction studies, simple means of dynamically adjusting the environmental viscoelasticity of cell cultures are needed. Here, a novel method is presented to dynamically and reversibly control the viscoelasticity of naturally derived polymer hydrogels through interactions with poly (ethylene glycol) (PEG). Interactions between PEG and hydrogel polymers, possibly involving hydrogen bonding, stiffen the hydrogel matrices. By dynamically changing the PEG concentration of the solution in which polymer hydrogels are incubated, their viscoelastic properties are adjusted, which in turn affects cell adhesion and cytoskeletal organization. Importantly, this effects is reversible, providing a cost\u2010effective and simple strategy for dynamically adjusting the viscoelasticity of polymer hydrogels. This method holds promise for applications in mechanobiology, biomedicine, and the life sciences.<\/jats:p>","DOI":"10.1002\/adma.202408616","type":"journal-article","created":{"date-parts":[[2025,2,12]],"date-time":"2025-02-12T00:45:30Z","timestamp":1739321130000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Dynamic\u00a0and Reversible\u00a0Tuning\u00a0of Hydrogel Viscoelasticity\u00a0by Transient Polymer Interactions for\u00a0Controlling Cell Adhesion"],"prefix":"10.1002","volume":"37","author":[{"given":"Shane","family":"Scott","sequence":"first","affiliation":[{"name":"Department of Materials Science and Engineering McMaster University  1280 Main St. W. 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