{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T22:00:16Z","timestamp":1791842416540,"version":"4.3.4"},"reference-count":59,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,1,2]],"date-time":"2025-01-02T00:00:00Z","timestamp":1735776000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013850","name":"Helmut Horten Stiftung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013850","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Materials"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>3D scaffolds enable biological investigations with a more natural cell conformation. However, the porosity of synthetic hydrogels is often limited to the nanometer scale, which confines the movement of 3D encapsulated cells and restricts dynamic cell processes. Precise control of hydrogel porosity across length scales remains a challenge and the development of porous materials that allow cell infiltration, spreading, and migration in a manner more similar to natural ECM environments is desirable. Here, a straightforward and reliable method is presented for generating kinetically\u2010controlled macroporous biomaterials using liquid\u2013liquid phase separation between poly(ethylene glycol) (PEG) and dextran. Photopolymerization\u2010induced phase separation resulted in macroporous hydrogels with tunable pore size. Varying light intensity and hydrogel composition controlled polymerization kinetics, time to percolation, and complete gelation, which defined the average pore diameter (\u00d8 = 1\u2013200 \u00b5m) and final gel stiffness of the formed hydrogels. Critically, for biological applications, macroporous hydrogels are prepared from aqueous polymer solutions at physiological pH and temperature using visible light, allowing for direct cell encapsulation. Human dermal fibroblasts in a range of macroporous gels are encapsulated with different pore sizes. Porosity improved cell spreading with respect to bulk gels and allowed migration in the porous biomaterials.<\/jats:p>","DOI":"10.1002\/adma.202410452","type":"journal-article","created":{"date-parts":[[2025,1,2]],"date-time":"2025-01-02T07:12:12Z","timestamp":1735801932000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Tunable Bicontinuous Macroporous Cell Culture Scaffolds via Kinetically Controlled Phase Separation"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9587-7053","authenticated-orcid":false,"given":"Oksana Y.","family":"Dudaryeva","sequence":"first","affiliation":[{"name":"Macromolecular Engineering Laboratory Department of Mechanical and Process Engineering ETH Zurich  Zurich 8092 Switzerland"},{"name":"Department of Orthopedics University Medical Center Utrecht  Utrecht 3584 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