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Unfortunately, a system offering ultrasensitive detection capabilities in a form that requires little auxiliary equipment or training has remained elusive. Here, a colorimetric DNAzyme\u2010crosslinked hydrogel sensor is presented. In the presence of a target pathogen, DNAzyme cleavage results in hydrogel dissolution, yielding the release of entrapped gold nanoparticles in a manner visible to the naked eye. Recognizing that\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    holds high relevance within both environmental and clinical environments, an\n                    <jats:italic>E. coli<\/jats:italic>\n                    \u2010responsive DNAzyme is incorporated into this platform. Through the optimization of the hydrogel polymerization process and the discovery of bacteriophage\u2010induced DNAzyme signal amplification, 10\n                    <jats:sup>1<\/jats:sup>\n                    CFU mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    <jats:italic>E. coli<\/jats:italic>\n                    is detected within real\u2010world lake water samples. Subsequent pairing with an artificial intelligence model removed ambiguity in sensor readout, offering 96% true positive and 100% true negative accuracy. Finally, high sensor specificity and stability results supported clinical use, where 100% of urine samples collected from patients with\n                    <jats:italic>E. coli<\/jats:italic>\n                    urinary tract infections are accurately identified. No false positives are observed when testing healthy samples. Ultimately, this platform stands to significantly improve population health by substantially increasing pathogen testing accessibility.\n                  <\/jats:p>","DOI":"10.1002\/adma.202411173","type":"journal-article","created":{"date-parts":[[2024,11,26]],"date-time":"2024-11-26T06:49:45Z","timestamp":1732603785000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Bacteriophage\u2010Activated DNAzyme Hydrogels Combined with Machine Learning Enable Point\u2010of\u2010Use Colorimetric Detection of\n                    <i>Escherichia coli<\/i>"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-9595-0569","authenticated-orcid":false,"given":"Hannah","family":"Mann","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering McMaster University  1280 Main Street West Hamilton ON L8S 4L8 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5088-3441","authenticated-orcid":false,"given":"Shadman","family":"Khan","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering McMaster University  1280 Main Street West Hamilton ON L8S 4L8 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2636-5097","authenticated-orcid":false,"given":"Akansha","family":"Prasad","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering McMaster University  1280 Main Street West Hamilton ON L8S 4L8 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8868-1650","authenticated-orcid":false,"given":"Fereshteh","family":"Bayat","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering McMaster University  1280 Main Street West Hamilton ON L8S 4L8 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4313-9523","authenticated-orcid":false,"given":"Jimmy","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biomedical Sciences McMaster University  1280 Main Street West Hamilton ON L8S 4L8 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1824-3604","authenticated-orcid":false,"given":"Kyle","family":"Jackson","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering McMaster University  1280 Main Street West Hamilton ON L8S 4L8 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7533-6743","authenticated-orcid":false,"given":"Yingfu","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biomedical Sciences McMaster University  1280 Main Street West Hamilton ON L8S 4L8 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9261-7611","authenticated-orcid":false,"given":"Zeinab","family":"Hosseinidoust","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering McMaster University  1280 Main Street West Hamilton ON L8S 4L8 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8757-8002","authenticated-orcid":false,"given":"Tohid F.","family":"Didar","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering McMaster University  1280 Main Street West Hamilton ON L8S 4L8 Canada"},{"name":"Department of Mechanical Engineering McMaster University  Hamilton ON L8S 4L7 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7410-3323","authenticated-orcid":false,"given":"Carlos D. 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