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However, the insufficient co\u2010stimulating molecules and loss of peptide\u2010major histocompatibility complex I (MHC\u2010I) expression on tumor cells lead to poor therapy of sonoimmunotherapies. Herein, this work develops a sonosensitive system to augment MHC\u2010I unrestricted natural killer (NK) cell\u2010mediated innate immunity and T cell\u2010mediated adaptive immunity by leveraging antigen presentation cell (APC)\u2010like tumor cells. Genetically engineered tumor cells featuring sufficient co\u2010stimulating molecules are cryo\u2010shocked and conjugated with a sonosensitizer, hematoporphyrin monomethyl ether, using click chemistry. These cells (DPNLs) exhibit characteristics of tumor and draining lymph node homing. Under ultrasound, NK cell\u2010mediated innate immunity within the tumor microenvironment could be activated, and T cells in the tumor\u2010draining lymph nodes (TDLNs) are stimulated through co\u2010stimulatory molecules. In combination with programmed cell death ligand 1 (PD\u2010L1) antibody, DPNLs extend the survival time and inhibited lung metastasis in triple\u2010negative breast cancer (TNBC) models. This study provides an alternative approach for sonoimmunotherapy with precise sonosensitizer delivery and enhanced NK cell and T cell activation.<\/jats:p>","DOI":"10.1002\/adma.202413289","type":"journal-article","created":{"date-parts":[[2025,2,16]],"date-time":"2025-02-16T11:06:39Z","timestamp":1739703999000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Cryo\u2010Shocked Tumor\u2010Reprogrammed Sonosensitive Antigen\u2010Presenting Cells Improving Sonoimmunotherapy via T Cells and NK Cells Immunity"],"prefix":"10.1002","volume":"37","author":[{"given":"Xindi","family":"Qian","sequence":"first","affiliation":[{"name":"Department of Medical Ultrasound and Center of Minimally Invasive Treatment for Tumor Shanghai Tenth People's Hospital Ultrasound Research and Education Institute School of Medicine Tongji University  Shanghai 200072 China"},{"name":"State Key Laboratory of Drug Research &amp; Center of Pharmaceutics Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai 201203 China"},{"name":"Department of Ultrasound Zhongshan Hospital Institute of Ultrasound in Medicine and Engineering Fudan University  Shanghai 200032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenzhe","family":"Yi","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Drug Research &amp; 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