{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T19:29:20Z","timestamp":1792092560700,"version":"4.4.0"},"reference-count":56,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T00:00:00Z","timestamp":1734480000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82174497"],"award-info":[{"award-number":["82174497"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81973945"],"award-info":[{"award-number":["81973945"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Materials"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Follicular tracking is typically conducted using ultrasound technology, but its effectiveness is constrained by limited resolution. High\u2010resolution imaging of deep tissues can be accomplished using luminescence imaging in the near\u2010infrared II window (NIR\u2010II, 1000\u20131700\u00a0nm); however, the contrast agents that are used lack specificity. Here, it is reported that the FDA\u2010approved indocyanine green (ICG)\u2010conjugated recombinant human chorionic gonadotropin (hCG) protein can target early follicles with long\u2010term effectiveness. A novel high\u2010resolution NIR\u2010II imaging approach is developed for monitoring follicular development as well as ovulation using multi\u2010color imaging of ovarian vessels with a combination of non\u2010overlapping downconversion nanoparticles (DCNPs). The results showed that the ability to monitor early follicles of around 50\u00a0\u00b5m in diameter exceeded the spatial and temporal resolution of ultrasound or MRI without the reproductive damage associated with computed tomography radiation, and this enabled the clinical identification of the follicular reserve in patients with infertility diseases such as polycystic ovary syndrome (PCOS). In addition, NIR\u2010II imaging clearly targeted ovarian tumors and showed micro\u2010metastatic lesions, thus providing a new tool for monitoring tumors in vivo and guiding surgical resection.<\/jats:p>","DOI":"10.1002\/adma.202414129","type":"journal-article","created":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T02:19:59Z","timestamp":1734574799000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Molecular Imaging of Ovarian Follicles and Tumors With Near\u2010Infrared II Bioconjugates"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9102-4363","authenticated-orcid":false,"given":"Yicong","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Integrative Medicine and Neurobiology School of Basic Medical Sciences Shanghai Medical College Brain Science Collaborative Innovation Center State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Institutes of Brain Science Fudan University  Shanghai 200032 China"},{"name":"Shanghai Key Laboratory of Acupuncture Mechanism and Acupoint Function Shanghai Institute of Acupuncture and Moxibustion  Shanghai 200433 China"},{"name":"Fudan Zhangjiang Institute  Shanghai 201203 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3890-5553","authenticated-orcid":false,"given":"Wenhan","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology &amp; Visual Science Eye &amp; ENT Hospital, Shanghai Medical College Fudan University  83 Fenyang Road Shanghai 200031 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3322-1489","authenticated-orcid":false,"given":"Zi\u2010Han","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Chemistry State Key Laboratory of Molecular Engineering of Polymers and iChem Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials Fudan University  Shanghai 200433 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-2281-4375","authenticated-orcid":false,"given":"Yan","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Integrative Medicine and Neurobiology School of Basic Medical Sciences Shanghai Medical College Brain Science Collaborative Innovation Center State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Institutes of Brain Science Fudan University  Shanghai 200032 China"},{"name":"Shanghai Key Laboratory of Acupuncture Mechanism and Acupoint Function Shanghai Institute of Acupuncture and Moxibustion  Shanghai 200433 China"},{"name":"Fudan Zhangjiang Institute  Shanghai 201203 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1077-9305","authenticated-orcid":false,"given":"Yu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Integrative Medicine and Neurobiology School of Basic Medical Sciences Shanghai Medical College Brain Science Collaborative Innovation Center State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Institutes of Brain Science Fudan University  Shanghai 200032 China"},{"name":"Shanghai Key Laboratory of Acupuncture Mechanism and Acupoint Function Shanghai Institute of Acupuncture and Moxibustion  Shanghai 200433 China"},{"name":"Fudan Zhangjiang Institute  Shanghai 201203 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7928-2678","authenticated-orcid":false,"given":"Wenhao","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Integrative Medicine and Neurobiology School of Basic Medical Sciences Shanghai Medical College Brain Science Collaborative Innovation Center State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Institutes of Brain Science Fudan University  Shanghai 200032 China"},{"name":"Shanghai Key Laboratory of Acupuncture Mechanism and Acupoint Function Shanghai Institute of Acupuncture and Moxibustion  Shanghai 200433 China"},{"name":"Fudan Zhangjiang Institute  Shanghai 201203 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0201-8912","authenticated-orcid":false,"given":"Zhiming","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Drug Research Molecular Imaging Center Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai 201203 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1606-9198","authenticated-orcid":false,"given":"Ruihu","family":"Song","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Drug Research Molecular Imaging Center Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai 201203 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2145-2551","authenticated-orcid":false,"given":"Zhao","family":"Fang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Drug Research Molecular Imaging Center Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai 201203 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8813-633X","authenticated-orcid":false,"given":"Wei","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Integrative Medicine and Neurobiology School of Basic Medical Sciences Shanghai Medical College Brain Science Collaborative Innovation Center State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Institutes of Brain Science Fudan University  Shanghai 200032 China"},{"name":"Shanghai Key Laboratory of Acupuncture Mechanism and Acupoint Function Shanghai Institute of Acupuncture and Moxibustion  Shanghai 200433 China"},{"name":"Fudan Zhangjiang Institute  Shanghai 201203 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2338-1367","authenticated-orcid":false,"given":"Xiaoyu","family":"Tong","sequence":"additional","affiliation":[{"name":"Department of Integrative Medicine and Neurobiology School of Basic Medical Sciences Shanghai Medical College Brain Science Collaborative Innovation Center State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Institutes of Brain Science Fudan University  Shanghai 200032 China"},{"name":"Shanghai Key Laboratory of Acupuncture Mechanism and Acupoint Function Shanghai Institute of Acupuncture and Moxibustion  Shanghai 200433 China"},{"name":"Fudan Zhangjiang Institute  Shanghai 201203 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2701-4037","authenticated-orcid":false,"given":"Kuinyu","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Integrative Medicine and Neurobiology School of Basic Medical Sciences Shanghai Medical College Brain Science Collaborative Innovation Center State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Institutes of Brain Science Fudan University  Shanghai 200032 China"},{"name":"Shanghai Key Laboratory of Acupuncture Mechanism and Acupoint Function Shanghai Institute of Acupuncture and Moxibustion  Shanghai 200433 China"},{"name":"Fudan Zhangjiang Institute  Shanghai 201203 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8921-2872","authenticated-orcid":false,"given":"Zhenle","family":"Pei","sequence":"additional","affiliation":[{"name":"Shanghai Ji Ai Genetics &amp; IVF Institute Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases Obstetrics and Gynecology Hospital of Fudan University  Shanghai 200011 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4011-8495","authenticated-orcid":false,"given":"Minzhen","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine Fudan University Shanghai Cancer Center  Shanghai 200032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7886-6144","authenticated-orcid":false,"given":"Fan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Chemistry State Key Laboratory of Molecular Engineering of Polymers and iChem Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials Fudan University  Shanghai 200433 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8470-5467","authenticated-orcid":false,"given":"Hao","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Drug Research Molecular Imaging Center Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai 201203 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7156-6023","authenticated-orcid":false,"given":"Yi","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Integrative Medicine and Neurobiology School of Basic Medical Sciences Shanghai Medical College Brain Science Collaborative Innovation Center State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Institutes of Brain Science Fudan University  Shanghai 200032 China"},{"name":"Shanghai Key Laboratory of Acupuncture Mechanism and Acupoint Function Shanghai Institute of Acupuncture and Moxibustion  Shanghai 200433 China"},{"name":"Fudan Zhangjiang Institute  Shanghai 201203 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,18]]},"reference":[{"key":"e_1_2_11_1_1","doi-asserted-by":"publisher","DOI":"10.1210\/clinem\/dgac039"},{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.09.020"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2003-031732"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23010512"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1006\/frne.1998.0167"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.01.030"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmri.23992"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmg044"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1312830110"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/09513590.2017.1306848"},{"key":"e_1_2_11_8_2","first-page":"164, 8","author":"Zhou Y.","year":"2023","journal-title":"Endocrinology"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deu353"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3728"},{"key":"e_1_2_11_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201705799"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2018.10.015"},{"key":"e_1_2_11_11_2","doi-asserted-by":"publisher","DOI":"10.1148\/radiol.2015132698"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0MH00870B"},{"key":"e_1_2_11_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2022.137848"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201900321"},{"key":"e_1_2_11_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmat4476"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-016-0010"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2020.10.267"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1BM00396H"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201800589"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.3c00100"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201800106"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.7b04211"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.50.070181.002341"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gau041"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(00)00340-3"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21708"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-05113-8"},{"key":"e_1_2_11_25_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.3c04949"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201705799"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241713030"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6SC04897H"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.5551\/jat.RV17018"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-006-0223-3"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1148\/radiol.2511081300"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-18051-1"},{"key":"e_1_2_11_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-03505-4"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s44222-022-00002-8"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202104567"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbon.2023.118728"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jre.2020.01.021"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3728"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/17.4.857"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1718917115"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10103-007-0524-0"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep12446"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.molpharmaceut.0c00437"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod48.6.1367"},{"key":"e_1_2_11_43_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo.132.5.8477671"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp0808697"},{"key":"e_1_2_11_44_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp1514916"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.10.010"}],"container-title":["Advanced Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adma.202414129","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T21:49:20Z","timestamp":1759787360000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adma.202414129"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,18]]},"references-count":56,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,2]]}},"alternative-id":["10.1002\/adma.202414129"],"URL":"https:\/\/doi.org\/10.1002\/adma.202414129","archive":["Portico"],"relation":{},"ISSN":["0935-9648","1521-4095"],"issn-type":[{"value":"0935-9648","type":"print"},{"value":"1521-4095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,18]]},"assertion":[{"value":"2024-09-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2414129"}}