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However, the application of base editors in cell manufacturing is hindered by inconsistent efficiency and high costs, contributed by suboptimal delivery methods. Nanoneedles have emerged as an effective delivery approach, enabling highly efficient, non\u2010perturbing gene therapies both in vitro and in vivo. Here we demonstrate that nanoneedle delivery of an adenine base editor corrects a heterozygous single\u2010nucleotide pathogenic variant in\n                    <jats:italic>COL7A1<\/jats:italic>\n                    in primary RDEB fibroblasts in vitro with 96.5% efficiency, without inducing off\u2010target variants. The nanoneedle delivery maintains cell viability and displays modest phenotypical alterations unlike conventional cationic lipid transfection. The nanoneedle\u2010mediated editing significantly increases the production and secretion of full\u2010length type VII collagen protein, contributing to restore functional fibroblasts phenotype by improving cell adhesion. These findings underscore the suitability and safety of nanoneedles for gene editing in a clinically relevant context of cell manufacturing, establishing a foundation for their use in cell therapies.\n                  <\/jats:p>","DOI":"10.1002\/adma.202414728","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T10:16:11Z","timestamp":1741774571000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Porous Silicon Nanoneedles Efficiently Deliver Adenine Base Editor to Correct a Recurrent Pathogenic\n                    <i>COL7A1<\/i>\n                    Variant in Recessive Dystrophic Epidermolysis Bullosa"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5414-4478","authenticated-orcid":false,"given":"Salman Ahmad","family":"Mustfa","sequence":"first","affiliation":[{"name":"Centre for Craniofacial and Regenerative Biology King's College London 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