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A major bottleneck in nanopore analysis is the fragility of the supporting lipid membranes, that easily rupture after exposure to biological samples. Membranes comprising PMOXA\u2010PDMS\u2010PMOXA (poly(2\u2010methyloxazoline\u2010b\u2010dimethylsiloxane\u2010b\u20102\u2010methyloxazoline)) or PBD\u2010PEO (poly(1,2\u2010butadiene)\u2010b\u2010poly(ethylene oxide)) polymers may form robust alternatives, but their suitability for the reconstitution of a broad range of nanopores has not yet been investigated. Here, PBD\u2010PEO membranes are found to be highly robust toward applied voltages and human serum, while providing a poor environment for nanopore reconstitution. However, hybrid membranes containing a similar molar ratio of PBD\n                    <jats:sub>11<\/jats:sub>\n                    PEO\n                    <jats:sub>8<\/jats:sub>\n                    polymers and diphytanoyl phosphatidylcholine (DPhPC) lipids show the best of both worlds: highly robust membranes suitable for the reconstitution of a wide variety of nanopores. Molecular dynamics simulations reveal that lipids form \u224812\u00a0nm domains interspersed by a polymer matrix. Nanopores partition into these lipid nanodomains and sequester lipids, possibly offering the same binding strength as in a native bilayer. Nanopores reconstituted in hybrid membranes yield efficient sampling of biomolecules and enable sensing of high concentrations of human serum. This work thus shows that hybrid membranes functionalized with nanopores allow single\u2010molecule sensing, while forming robust interfaces, resolving an important bottleneck for novel nanopore\u2010based biosensors.\n                  <\/jats:p>","DOI":"10.1002\/adma.202418462","type":"journal-article","created":{"date-parts":[[2025,3,8]],"date-time":"2025-03-08T03:33:31Z","timestamp":1741404811000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Nanopore\u2010Functionalized Hybrid Lipid\u2010Block Copolymer Membranes Allow Efficient Single\u2010Molecule Sampling and Stable Sensing of Human Serum"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4198-6745","authenticated-orcid":false,"given":"Edo","family":"Vreeker","sequence":"first","affiliation":[{"name":"Groningen Biomolecular Sciences and Biotechnology Institute University of Groningen  Nijenborg 7 Groningen 9747 AG The 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