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Here, a simple strategy is introduced that utilizes charge repulsion between graphene oxide (GO) and engineered bacteria to induce liquid crystal formation, enabling their layer\u2010by\u2010layer self\u2010assembly on a polyethersulfone membrane. The interlayer pressure flattens the bacteria, removing interlayer water and forming a densely packed structure. This compression decreases the spacing between functional groups, leading to a robust hydrogen bonding network and a significant enhancement in mechanical properties (12.42 times tensile strength increase). Notably, the pressure preserves the activity of the super uranyl\u2010binding protein of engineered bacteria, which selectively coordinates with uranyl (UO\n                    <jats:sub>2<\/jats:sub>\n                    <jats:sup>2+<\/jats:sup>\n                    ) through high\u2010affinity coordination bonds, enabling recognition and sieving of target ions. The membrane demonstrates near 100% rejection of UO\n                    <jats:sub>2<\/jats:sub>\n                    <jats:sup>2+<\/jats:sup>\n                    , K\/U, and V\/U selectivity of \u2248140 and \u224840, respectively, while maintaining long\u2010term stability. This strategy provides a versatile platform for the precise design of high\u2010performance membranes, advancing the field of molecular transport in energy and environmental applications.\n                  <\/jats:p>","DOI":"10.1002\/adma.202419496","type":"journal-article","created":{"date-parts":[[2025,2,13]],"date-time":"2025-02-13T04:57:27Z","timestamp":1739422647000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["2D Biomimetic Membranes Constructed by Charge Assembly and Hydrogen Bonding for Precise Ion Separation"],"prefix":"10.1002","volume":"37","author":[{"given":"Zixiao","family":"Lv","sequence":"first","affiliation":[{"name":"Frontiers Science Center for Rare Isotopes Lanzhou University  Tianshui South Road 222 Lanzhou 730000 China"},{"name":"School of Nuclear Science and Technology Lanzhou University  Tianshui South Road 222 Lanzhou 730000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haidong","family":"Li","sequence":"additional","affiliation":[{"name":"Frontiers Science Center for Rare Isotopes Lanzhou University  Tianshui South Road 222 Lanzhou 730000 China"},{"name":"School of Nuclear Science and Technology Lanzhou University  Tianshui South Road 222 Lanzhou 730000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanxi","family":"Wen","sequence":"additional","affiliation":[{"name":"Frontiers Science Center for Rare Isotopes Lanzhou University  Tianshui South Road 222 Lanzhou 730000 China"},{"name":"School of Nuclear Science and Technology Lanzhou University  Tianshui South Road 222 Lanzhou 730000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9343-6561","authenticated-orcid":false,"given":"Longlong","family":"Tian","sequence":"additional","affiliation":[{"name":"Frontiers Science Center for Rare Isotopes Lanzhou University  Tianshui South Road 222 Lanzhou 730000 China"},{"name":"School of Nuclear Science and Technology Lanzhou University  Tianshui South Road 222 Lanzhou 730000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"XiMeng","family":"Chen","sequence":"additional","affiliation":[{"name":"Frontiers Science Center for Rare Isotopes Lanzhou University  Tianshui South Road 222 Lanzhou 730000 China"},{"name":"School of Nuclear Science and Technology Lanzhou University  Tianshui South Road 222 Lanzhou 730000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wangsuo","family":"Wu","sequence":"additional","affiliation":[{"name":"Frontiers Science Center for Rare Isotopes Lanzhou University  Tianshui South Road 222 Lanzhou 730000 China"},{"name":"School of Nuclear Science and Technology Lanzhou University  Tianshui South Road 222 Lanzhou 730000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7748-2691","authenticated-orcid":false,"given":"Zhan","family":"Li","sequence":"additional","affiliation":[{"name":"Frontiers Science Center for Rare Isotopes Lanzhou University  Tianshui South Road 222 Lanzhou 730000 China"},{"name":"School of Nuclear Science and Technology Lanzhou University  Tianshui South Road 222 Lanzhou 730000 China"},{"name":"School of Chemistry and Chemical Engineering Qinghai Nationalities University  No. 3, Bayi Middle Road Xining 810007 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,13]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.0c05701"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202406535"},{"key":"e_1_2_8_3_1","volume":"12","author":"Casas X.","year":"2020","journal-title":"ACS Appl. 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