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The construction of artificial cells containing MLOs with enhanced complexity and functions is still challenging. Here a light\u2010responsive protein, Cry2olig\u2010IDRs, is designed and purified to form MLOs upon light (488\u00a0nm) irradiation. They are capable of rapidly recruiting positively charged inducible nitric oxide synthase (iNOS\n                    <jats:sup>+<\/jats:sup>\n                    ) from surroundings to regulate its activity for NO production. NO\u2010artificial cells are constructed by encapsulating Cry2olig\u2010IDRs and iNOS\n                    <jats:sup>+<\/jats:sup>\n                    into giant unilamellar vesicles, which are capable of rapid production of NO with high concentration due to the formation of MLOs upon light irradiation. NO\u2010artificial cells are confirmed to possess the ability for melanoma tumor therapy in mice. These findings provide an efficient method for remotely regulating enzyme activity inside artificial cells, paving the path to build more sophisticated artificial cells for their biomedical applications.\n                  <\/jats:p>","DOI":"10.1002\/adma.202500242","type":"journal-article","created":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T04:02:24Z","timestamp":1746504144000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Artificial Cells Capable of NO\u00a0Generation with Light Controllable Membraneless Organelles for Melanoma Therapy"],"prefix":"10.1002","volume":"37","author":[{"given":"Yingming","family":"Zhao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Urban Water Resource and Environment MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage School of Chemistry and Chemical Engineering Harbin Institute of Technology  No. 92, West 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