{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T19:43:55Z","timestamp":1792957435779,"version":"4.5.0"},"reference-count":57,"publisher":"Wiley","issue":"37","license":[{"start":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T00:00:00Z","timestamp":1750982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T00:00:00Z","timestamp":1750982400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22404029"],"award-info":[{"award-number":["22404029"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82471500"],"award-info":[{"award-number":["82471500"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32472651"],"award-info":[{"award-number":["32472651"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2024A1515011884"],"award-info":[{"award-number":["2024A1515011884"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Materials"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Alzheimer's disease (AD) is a progressive neurodegenerative disorder with no cure, making early diagnosis critical for mitigating its impact. Blood extracellular vesicles (EVs) hold promises as biomarkers for AD diagnosis, but current detection technologies lack the sensitivity and multiplexing capabilities needed for efficient diagnosis. Here, a novel label\u2010free bioelectronic platform is presented based on an organic electrochemical transistor (OECT) integrated with a microelectrode array (MEA) for ultrasensitive detection of AD biomarkers in blood EVs, including amyloid\u2010\u03b2 (A\u03b2\n                    <jats:sub>1\u201040<\/jats:sub>\n                    and A\u03b2\n                    <jats:sub>1\u201042<\/jats:sub>\n                    ), total tau (t\u2010tau), and phosphorylated tau (p\u2010tau\n                    <jats:sup>181<\/jats:sup>\n                    ). This platform achieves a detection limit as low as the zeptomolar (zM) level, enabling the detection of single\u2010molecule targets. It provides a comprehensive multiplexed diagnostic model capable of delivering results within 20 min. Notably, the systematic integration of multiple AD biomarkers in blood EVs is demonstrated to significantly enhance diagnostic accuracy. This study presents a novel EVs\u2010based multiplexed diagnostic model for AD, correctly classifying all clinical samples (\n                    <jats:italic>n<\/jats:italic>\n                    = 40), far exceeding the accuracy of a single biomarker. With its high sensitivity and rapid turnaround, this platform enables reliable AD diagnosis and holds the potential for tracking disease progression, offering a transformative tool to combat the societal burden of AD.\n                  <\/jats:p>","DOI":"10.1002\/adma.202505262","type":"journal-article","created":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T09:54:52Z","timestamp":1751018092000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Clinically Accurate Diagnosis of Alzheimer's Disease via Single\u2010Molecule Bioelectronic Label\u2010Free Profiling of Multiple Blood Extracellular Vesicle Biomarkers"],"prefix":"10.1002","volume":"37","author":[{"given":"Jintao","family":"Zheng","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Food Engineering Guangdong Technion\u2010Israel Institute of Technology (GTIIT)  Shantou 515063 China"},{"name":"Faculty of Biotechnology and Food Engineering Technion\u2010Israel Institute of Technology (IIT)  Haifa 3200003 Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohong","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Food Engineering Guangdong Technion\u2010Israel Institute of Technology (GTIIT)  Shantou 515063 China"},{"name":"Faculty of Biotechnology and Food Engineering Technion\u2010Israel Institute of Technology (IIT)  Haifa 3200003 Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiyao","family":"Bai","sequence":"additional","affiliation":[{"name":"School of Medicine The Chinese University of Hong Kong (CUHK)  Shenzhen 518172 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minchao","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Neurology The First Affiliated Hospital of Shantou University Medical College  Shantou 515041 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiacheng","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Food Engineering Guangdong Technion\u2010Israel Institute of Technology (GTIIT)  Shantou 515063 China"},{"name":"Faculty of Biotechnology and Food Engineering Technion\u2010Israel Institute of Technology (IIT)  Haifa 3200003 Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxi","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Food Engineering Guangdong Technion\u2010Israel Institute of Technology (GTIIT)  Shantou 515063 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Runzhi","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Food Engineering Guangdong Technion\u2010Israel Institute of Technology (GTIIT)  Shantou 515063 China"},{"name":"Faculty of Biotechnology and Food Engineering Technion\u2010Israel Institute of Technology (IIT)  Haifa 3200003 Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Food Engineering Guangdong Technion\u2010Israel Institute of Technology (GTIIT)  Shantou 515063 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoyang","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Food Engineering Guangdong Technion\u2010Israel Institute of Technology (GTIIT)  Shantou 515063 China"},{"name":"Faculty of Biotechnology and Food Engineering Technion\u2010Israel Institute of Technology (IIT)  Haifa 3200003 Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Food Engineering Guangdong Technion\u2010Israel Institute of Technology (GTIIT)  Shantou 515063 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dian","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine Shantou University Medical College (SUMC)  Shantou 515041 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuyan","family":"Wu","sequence":"additional","affiliation":[{"name":"Shantou University Medical College (SUMC)  Shantou 515041 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Liu","sequence":"additional","affiliation":[{"name":"Sichuan Provincial Key Laboratory for Human Disease Gene Study Department of Medical Genetics Sichuan Academy of Medical Sciences &amp; Sichuan Provincial People's Hospital University of Electronic Science and Technology of China  Chengdu 610072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenzhong","family":"Li","sequence":"additional","affiliation":[{"name":"School of Medicine The Chinese University of Hong Kong (CUHK)  Shenzhen 518172 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinguang","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tobacco Pest Monitoring Controlling and Integrated Management Tobacco Research Institute of Chinese Academy of Agricultural Sciences  Qingdao 266101 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Chongqing General Hospital School of Medicine Chongqing University  Chongqing 400417 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Jiang","sequence":"additional","affiliation":[{"name":"School of Medicine The Chinese University of Hong Kong (CUHK)  Shenzhen 518172 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6383-6321","authenticated-orcid":false,"given":"Keying","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Food Engineering Guangdong Technion\u2010Israel Institute of Technology (GTIIT)  Shantou 515063 China"},{"name":"Faculty of Biotechnology and Food Engineering Technion\u2010Israel Institute of Technology (IIT)  Haifa 3200003 Israel"},{"name":"Guangdong Provincial Key Laboratory of Materials and Technologies for Energy Conversion Guangdong Technion\u2010Israel Institute of Technology (GTIIT)  Shantou 515063 China"},{"name":"Monash Institute of Pharmaceutical Sciences (MIPS) Monash University  Parkville Victoria 3052 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,27]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43587-023-00404-2"},{"key":"e_1_2_8_2_1","first-page":"1797","volume":"28","author":"Mil\u00e0\u2010Alom\u00e0 M.","year":"2022","journal-title":"Nat. Med."},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/alz.12694"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41582-023-00789-z"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43587-023-00403-3"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-021-01382-x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2021.101544"},{"key":"e_1_2_8_8_1","first-page":"3392","volume":"30","author":"O'Leary K.","year":"2024","journal-title":"Nat. Med."},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43587-024-00731-y"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-024-02869-z"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2024.151644"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2024.135714"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jalz.2019.05.002"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2022.114935"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201900613"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.2c05751"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-24319-x"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abm3088"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.4c05329"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2022.116605"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-020-00460-7"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.201900925"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40820-021-00753-w"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2023.117198"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaneurol.2020.4200"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-44818-x"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/alz.12166"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aau6977"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40035-017-0072-x"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2021.04.020"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.20517\/evcna.2023.72"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.4c02619"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13195-022-01010-x"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/alz.13823"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/alz.14497"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.15112"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2CS00920J"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41578-024-00652-7"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41563-020-0638-3"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/aelm.202400474"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202305347"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/natrevmats.2017.86"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/s44222-024-00180-7"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.4c12892"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-021-00734-9"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202201178"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.0c09893"},{"key":"e_1_2_8_48_1","first-page":"5698","volume":"18","author":"Albarghouthi F. M.","year":"2024","journal-title":"ACS Nano"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202304102"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-05235-z"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2022.114224"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/aenm.202003626"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-11534-w"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-47286-5"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awad361"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1255555"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2022.114365"}],"container-title":["Advanced Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adma.202505262","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/adma.202505262","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adma.202505262","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T22:01:03Z","timestamp":1771279263000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adma.202505262"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,27]]},"references-count":57,"journal-issue":{"issue":"37","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["10.1002\/adma.202505262"],"URL":"https:\/\/doi.org\/10.1002\/adma.202505262","archive":["Portico"],"relation":{},"ISSN":["0935-9648","1521-4095"],"issn-type":[{"value":"0935-9648","type":"print"},{"value":"1521-4095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,27]]},"assertion":[{"value":"2025-03-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2505262"}}