{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T11:46:05Z","timestamp":1782733565010,"version":"3.54.5"},"reference-count":65,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T00:00:00Z","timestamp":1782691200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T00:00:00Z","timestamp":1782691200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000196","name":"Canada Foundation for Innovation","doi-asserted-by":"publisher","award":["G248924"],"award-info":[{"award-number":["G248924"]}],"id":[{"id":"10.13039\/501100000196","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["G247765"],"award-info":[{"award-number":["G247765"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Materials"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Thrombotic disorders are a leading cause of cardiovascular mortality worldwide; however, real\u2010time, point\u2010of\u2010care monitoring technologies for timely detection of evolving coagulopathies remain inaccessible. Wearable, minimally invasive tracking of thrombo\u2010inflammatory activity could enable earlier risk assessment and more effective therapy monitoring than conventional episodic blood tests. Here, we present a reagent\u2010free, wearable electrochemical platform that integrates an on\u2010chip Prussian Blue (PB) redox transducer with a signal\u2010off molecularly imprinted polymer (MIP) layer and a biocompatible hydrogel microneedle (HMN) array for interstitial fluid (ISF) sampling, enabling direct electrochemical detection of thrombo\u2010inflammatory biomarkers. The electrochemical PB\/MIP (e\u2010MIP) biosensor was configured to quantify thrombin (thrombotic biomarker) as well as interleukin\u20106 (IL\u20106) and tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1) (inflammatory biomarkers) directly in dermal ISF extracted via the integrated HMNs. The wearable e\u2010MIP was characterized in vitro and ex vivo, where porcine skin tests preserved linearity and achieved limits of detection (LODs) of 0.26 ng mL\n                    <jats:sup>\u2212<\/jats:sup>\n                    <jats:sup>1<\/jats:sup>\n                    for thrombin and \u2264 0.41 pg mL\n                    <jats:sup>\u2212<\/jats:sup>\n                    <jats:sup>1<\/jats:sup>\n                    for IL\u20106 and TNF\u2010\u03b1, confirming sensitive performance in a skin model. Also, selectivity studies against potential interferents (e.g., prothrombin or cardiac troponins) were conducted to assess the possible cross\u2010reactivity. in vivo, HMN\u2010integrated patches applied to lipopolysaccharide (LPS)\u2013challenged rats sampled ISF and delivered it to the e\u2010MIP, which captured the rise\u2010and\u2010fall kinetics of thrombin and IL\u20106 over 0\u201324 h. The results were validated against parallel enzyme\u2010linked immunosorbent assays (ELISAs) performed on plasma collected at corresponding time points. Its versatile architecture and demonstrated in vivo performance position it as a promising platform that can enable early thrombotic risk assessment and therapeutic monitoring, with potential applications in personalized cardiovascular management following clinical validation.\n                  <\/jats:p>","DOI":"10.1002\/adma.202521589","type":"journal-article","created":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T11:13:58Z","timestamp":1782731638000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["In Vivo Monitoring of Thrombo\u2010Inflammatory Biomarkers via Molecularly Imprinted Polymer\u2010Integrated Hydrogel Microneedles"],"prefix":"10.1002","author":[{"given":"Mahmoud Ayman","family":"Saleh","sequence":"first","affiliation":[{"name":"Department of Bioengineering McGill University  Montreal Quebec 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