{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T18:14:30Z","timestamp":1789582470047,"version":"build-2803163510"},"reference-count":46,"publisher":"Wiley","issue":"44","license":[{"start":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T00:00:00Z","timestamp":1778198400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T00:00:00Z","timestamp":1778198400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004412","name":"T\u00fcrkiye Bilimler Akademisi","doi-asserted-by":"publisher","award":["GEBIP Award"],"award-info":[{"award-number":["GEBIP Award"]}],"id":[{"id":"10.13039\/501100004412","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008967","name":"Bilim Akademisi","doi-asserted-by":"publisher","award":["BAGEP Award"],"award-info":[{"award-number":["BAGEP Award"]}],"id":[{"id":"10.13039\/501100008967","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100024335","name":"\u0130zmir Biyot\u0131p ve Genom Merkezi, Dokuz Eyl\u00fcl \u00dcniversitesi","doi-asserted-by":"publisher","award":["Science Award"],"award-info":[{"award-number":["Science Award"]}],"id":[{"id":"10.13039\/501100024335","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Materials"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Extracellular vesicles (EVs) are nanoscale lipid\u2010bilayered structures that mediate intercellular communications by transporting nucleic acids, proteins, and lipids across diverse biological fluids. Their diagnostic potential is immense, yet their heterogeneity poses persistent challenges for isolation and characterization, often leading to low yield, co\u2010isolation of contaminants, and vesicle damage. Here, we present a thermoresponsive polymer\u2013integrated plasmonic metasurface sensor that enables spatiotemporally controlled, label\u2010free EV isolation. The metasurface, engineered by repurposing nanograted optical disks, was functionalized with poly(\n                    <jats:italic>N<\/jats:italic>\n                    \u2010isopropylacrylamide) (PNIPAM) and anti\u2010CD63 antibodies to achieve selective EV capture at physiological temperature and gentle release upon a minute thermal change near the polymer's lower critical solution temperature (\u223c35\u00b0C). Using MCF\u20107 and HEK\u2010293\u2013derived EVs as a proof\u2010of\u2010concept, the platform exhibited a dynamic detection range spanning three orders of magnitude. Release efficiency reached 87.03 \u00b1 23.5%, while both nanoparticle tracking analysis (NTA) and fluorescent NTA (fNTA) revealed up to \u223c100\u2010fold increase in EV purity relative to the ultrafiltration process. Electron microscopy and Western blotting confirmed preserved vesicle morphology and marker expression. By integrating thermoresponsive chemistry with a cost\u2010effective metasurface platform, this system offers a non\u2010destructive, portable, and real\u2010time solution for precise EV manipulation, advancing EV\u2010focused biosensing and point\u2010of\u2010care strategies for liquid biopsy applications in the future.\n                  <\/jats:p>","DOI":"10.1002\/adma.202522964","type":"journal-article","created":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T12:55:24Z","timestamp":1778244924000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Thermally Modulated Metasurface Sensor for Dynamic and Time\u2010Resolved Isolation of Extracellular Vesicles"],"prefix":"10.1002","volume":"38","author":[{"given":"Beyza Nur","family":"Kucuk","sequence":"first","affiliation":[{"name":"UNAM\u2014National Nanotechnology Research Center Bilkent University  Ankara Turkey"},{"name":"Institute of Materials Science and Nanotechnology Bilkent University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eylul Gulsen","family":"Yilmaz","sequence":"additional","affiliation":[{"name":"UNAM\u2014National Nanotechnology Research Center Bilkent University  Ankara Turkey"},{"name":"Institute of Materials Science and Nanotechnology Bilkent University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuf","family":"Aslan","sequence":"additional","affiliation":[{"name":"UNAM\u2014National Nanotechnology Research Center Bilkent University  Ankara Turkey"},{"name":"Institute of Materials Science and Nanotechnology Bilkent University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emre","family":"Ece","sequence":"additional","affiliation":[{"name":"UNAM\u2014National Nanotechnology Research Center Bilkent University  Ankara Turkey"},{"name":"Institute of Materials Science and Nanotechnology Bilkent University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00d6zgecan","family":"Erdem","sequence":"additional","affiliation":[{"name":"UNAM\u2014National Nanotechnology Research Center Bilkent University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Murat Alp","family":"Gungen","sequence":"additional","affiliation":[{"name":"UNAM\u2014National Nanotechnology Research Center Bilkent University  Ankara Turkey"},{"name":"Institute of Materials Science and Nanotechnology Bilkent University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bengi \u00d6zg\u00fcn","family":"\u00d6zt\u00fcrk","sequence":"additional","affiliation":[{"name":"Faculty of Science Department of Chemistry Hacettepe University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9918-5038","authenticated-orcid":false,"given":"Fatih","family":"Inci","sequence":"additional","affiliation":[{"name":"UNAM\u2014National Nanotechnology Research Center Bilkent University  Ankara Turkey"},{"name":"Institute of Materials Science and Nanotechnology Bilkent University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.7b00534"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph14101050"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells13010095"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964\u2010023\u201001103\u20106"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12404"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2018.1535750"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsabm.4c00782"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2019.03.014"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241612704"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2021.113879"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2025.117275"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2021.107814"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtha.2024.01.010"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s44330\u2010024\u201000007\u20102"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.74305"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.langmuir.1c03119"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2022.114487"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/ar5001617"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/polym14112164"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/ACSSENSORS.4C02070"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011\u2010014\u20109972\u20100"},{"key":"e_1_2_8_23_1","unstructured":"Culture Collections \u201cCell Line Profile MCF \u201d7n.d."},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.25122\/jml\u20102021\u20101101"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2007.070152"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2018.1535750"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2011.05.013"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtice.2018.07.045"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-382239-0.00005-4"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01937"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.8b00993"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1134\/S0965545X2135011X"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/s19153354"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2022.131657"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165\u2010022X(03)00150\u20107"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matpr.2023.04.460"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2015.02.030"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2020.115898"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtbio.2022.100285"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.langmuir.4c03975"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.langmuir.4c04859"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtchem.2024.102232"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.5c07175"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476\u20104598\u201013\u2010134"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25235585"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrep.2021.101034"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-025-08366-8"}],"container-title":["Advanced Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adma.202522964","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/adma.202522964","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adma.202522964","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T06:33:50Z","timestamp":1786430030000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adma.202522964"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,8]]},"references-count":46,"journal-issue":{"issue":"44","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/adma.202522964"],"URL":"https:\/\/doi.org\/10.1002\/adma.202522964","archive":["Portico"],"relation":{},"ISSN":["0935-9648","1521-4095"],"issn-type":[{"value":"0935-9648","type":"print"},{"value":"1521-4095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,8]]},"assertion":[{"value":"2025-11-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e22964"}}