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The CRISPR\/Cas12a\u2010based diagnostic technology, despite its robustness as a promising tool, is constrained by suboptimal detection speed and sensitivity. Here we designed triblock DNA\u2010mediated spherical nucleic acids (tSNA) that acts as a spatially confined reporter with critical coupling distances between substrates, enabling Cas12a protein to rapidly identify concentrated and stretched single\u2010stranded substrates with size\u2010matching intervals. Precise control of distances on tSNA of varying sizes revealed a direct correlation between trans\u2010cleavage efficiency and coupling distance, indicating that only when the distance exceeds the protein size can it offer an appropriate reaction space. It demonstrates a rapid \u201cscooting\u201d reaction model on tSNA, resulting in a trans\u2010cleavage rate of 10\u00a0nm A30\u2010tSNA 12 times faster and a sensitivity that is two orders of magnitude higher than that in bulk solution. Furthermore, tSNA can serve as a novel recognition and colorimetric element in lateral\u2010flow strips, thereby reducing the detection time for pathogen nucleic acids to just 3 min. This \u201csize\u2010matching\u201d model of tSNA offers a new perspective on the regulation of Cas12a enzymatic activity, establishing a versatile platform to advance diagnostic development through ultrafast, CRISPR\u2010powered POC systems.<\/jats:p>","DOI":"10.1002\/adma.202523381","type":"journal-article","created":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T11:20:30Z","timestamp":1771327230000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Accelerated CRISPR\/Cas12a\u2010Based Point\u2010of\u2010Care Diagnostics Through Critical Coupling Distance Control"],"prefix":"10.1002","volume":"38","author":[{"given":"Tong","family":"Su","sequence":"first","affiliation":[{"name":"State Key Laboratory of Flexible Electronics (LoFE) &amp; Jiangsu Key Laboratory of Smart Biomaterials and Theranostic Technology Institute of Advanced Materials (IAM) Nanjing University of Posts and Telecommunications  Nanjing 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