{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T08:13:59Z","timestamp":1790151239216,"version":"4.0.1"},"reference-count":51,"publisher":"Wiley","issue":"52","license":[{"start":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T00:00:00Z","timestamp":1783814400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T00:00:00Z","timestamp":1783814400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Materials"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Insect olfactory receptors have evolved to detect a wide range of chemicals and biomolecules, offering a promising foundation for next\u2010generation chemical sensors. We report the first direct integration of the odorant receptor MhOR5 from\n                    <jats:italic>Machilis hrabei<\/jats:italic>\n                    onto a graphene field\u2010effect transistor (gFET) for selective, label\u2010free detection of small organic compounds (SOCs). Large scale production of MhOR5 was achieved for the first time, yielding a stable tetrameric protein (80% purity) with up to 3\u2010month stability. High\u2010quality gFETs were fabricated at the wafer scale and quality controlled before utilization, demonstrating highly reproducible chips. The MhOR5\u2010functionalized gFETs were tested against sixteen chemically diverse SOCs, including eugenol, DEET, hexanol, and octanol, across a concentration range of 200 n\n                    <jats:sc>m<\/jats:sc>\n                    to 10 m\n                    <jats:sc>m<\/jats:sc>\n                    . Each analyte induced a concentration\u2010dependent response in the sensor. Binding analysis within the tested SOCs revealed the strongest affinity for acetophenone (logK\n                    <jats:sub>D<\/jats:sub>\n                    = 0.28), and the weakest for sulcatone (logK\n                    <jats:sub>D<\/jats:sub>\n                    = 4.4). Notably, the sensor distinguished eugenol from its structural isomer, isoeugenol, an achievement difficult to detect using conventional sensors. This work establishes a modular biosensing platform that couples the molecular selectivity of insect olfactory receptors with the exceptional sensitivity of graphene\u2010based sensors. The MhOR5\u2010gFET biosensor demonstrates significant potential for applications in biotechnology, diagnostics, and biosurveillance.\n                  <\/jats:p>","DOI":"10.1002\/adma.74079","type":"journal-article","created":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T06:54:06Z","timestamp":1783925646000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Graphene Nanosensor for the Detection of Small Organic Compounds Using an Insect Olfactory Receptor"],"prefix":"10.1002","volume":"38","author":[{"given":"Deependra Kumar","family":"Ban","sequence":"first","affiliation":[{"name":"Shu Chien\u2010Gene Lay Department of Bioengineering Jacobs School of Engineering Department of Bioengineering University of California  San Diego California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicia","family":"Contet","sequence":"additional","affiliation":[{"name":"Eurofins CALIXAR  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Catania","sequence":"additional","affiliation":[{"name":"CardeaBio Inc. (Now Paragraf Limited)  San Diego California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerstin","family":"Kanonenberg","sequence":"additional","affiliation":[{"name":"Eurofins CALIXAR  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Tyler","sequence":"additional","affiliation":[{"name":"Paragraf Limited, Somersham  Cambridgeshire UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brett","family":"Goldsmith","sequence":"additional","affiliation":[{"name":"CardeaBio Inc. (Now Paragraf Limited)  San Diego California USA"},{"name":"Naval Information Warfare Center Pacific  San Diego California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5641-7284","authenticated-orcid":false,"given":"Kiana","family":"Aran","sequence":"additional","affiliation":[{"name":"Shu Chien\u2010Gene Lay Department of Bioengineering Jacobs School of Engineering Department of Bioengineering University of California  San Diego California USA"},{"name":"Department of Medicine,\u00a0Division of Geriatrics, Gerontology &amp; Palliative\u00a0Care,\u00a0School of Medicine University of California  San Diego California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,12]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph17186506"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1515\/REVEH.2007.22.1.39"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1TB00897H"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph182413147"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u20100668.1991.00001.x"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201068622\u2010x"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/s23115302"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1063\/5.0132177"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adl2882"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2011.11.003"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.3c00052"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.4c01177"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/nl301714x"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.8b19433"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.171001"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1635157100"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092\u20108674(00)80581\u20104"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0058889"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441\u2010020\u201003363\u2010x"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani11082463"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010025\u201087136\u2010y"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08834"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-801694-7.00006-8"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fevo.2015.00053"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018\u2010017\u20102627\u20105"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1017963108"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fevo.2015.00105"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2024.111317"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-323-91157-3.00002-7"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2009.04.035"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010021\u201003794\u20108"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1102896"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmat1967"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.1c09985"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.2c03399"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551\u2010019\u20100371\u2010x"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551\u2010021\u201000706\u2010z"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/adbi.202000594"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1089\/genbio.2024.0043"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/admt.202201945"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010019\u201038700\u2010w"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2013.46"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.200901173"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010019\u201050412\u20109"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1SD00036E"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2015.03.010"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2008.07.001"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2012.08.035"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2011.44"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2018.06.010"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1088\/0957\u20104484\/26\/12\/125502"}],"container-title":["Advanced Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adma.74079","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/adma.74079","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adma.74079","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T07:57:49Z","timestamp":1790150269000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adma.74079"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,12]]},"references-count":51,"journal-issue":{"issue":"52","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1002\/adma.74079"],"URL":"https:\/\/doi.org\/10.1002\/adma.74079","archive":["Portico"],"relation":{},"ISSN":["0935-9648","1521-4095"],"issn-type":[{"value":"0935-9648","type":"print"},{"value":"1521-4095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,12]]},"assertion":[{"value":"2026-02-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e74079"}}