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Probiotic biofilms offer a promising alternative to conventional antimicrobial coatings, but clinical translation is hindered by the lack of a stable, substrate\u2010generalizable immobilization method. Here we presented a broadly applicable \u201canchor\u2010and\u2010lock\u201d strategy that covalently tethered probiotic biofilms to diverse implants via bioorthogonal SPAAC reaction. A mussel\u2010inspired adhesive peptide functionalized with DBCO served as the surface anchor, while\n                    <jats:italic>L. casei<\/jats:italic>\n                    metabolically labeled with azide groups became the click\u2010ready lock. The resulting coatings formed uniformly on titanium, polyurethane, silicone rubber, and polypropylene, exhibiting long\u2010term stability. The biofilm operated through a dual\u2010phase antimicrobial mechanism, including an initial bactericidal phase (\u226596% killing of\n                    <jats:italic>S. aureus<\/jats:italic>\n                    ) mediated by lactic acid and hydrogen peroxide release, followed by a sustained anti\u2010adhesive phase that prevented pathogen colonization. RNA\u2010sequencing revealed that the coating downregulated\n                    <jats:italic>S. aureus<\/jats:italic>\n                    adhesion\u2010associated genes while suppressing carbohydrate transport and metabolic pathways. In clinically relevant delayed\u2010infection models in bone and bladder, functionalized implants prevented infection, mitigated inflammation, and promoted tissue integration, effects absent on unmodified controls. This SPAAC\u2010mediated strategy offers a broad approach to mitigating implant\u2010associated infections.\n                  <\/jats:p>","DOI":"10.1002\/adma.74083","type":"journal-article","created":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T08:21:23Z","timestamp":1784794883000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Bioorthogonal Anchor\u2010and\u2010Lock of Probiotic Biofilms on Diverse Biomaterials to Combat Delayed Implant\u2010Associated Infection"],"prefix":"10.1002","volume":"38","author":[{"given":"Meng","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Materials Science and Engineering South China University of Technology  Guangzhou China"},{"name":"National Engineering Research Center for Tissue Restoration and Reconstruction South China University of Technology  Guangzhou China"},{"name":"Guangdong Provincial Key Laboratory of Biomedical Engineering South China 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinchun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Guanghua School of Stomatology Guangdong Provincial Key Laboratory of Stomatology Hospital of Stomatology Sun Yat\u2010sen University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1880-1143","authenticated-orcid":false,"given":"Lin","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Materials Science and Engineering South China University of Technology  Guangzhou China"},{"name":"National Engineering Research Center for Tissue Restoration and Reconstruction South China University of Technology  Guangzhou China"},{"name":"Guangdong Provincial Key Laboratory of Biomedical Engineering South China University of Technology  Guangzhou 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