{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T17:34:48Z","timestamp":1791653688152,"version":"4.3.4"},"reference-count":82,"publisher":"Wiley","issue":"55","license":[{"start":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T00:00:00Z","timestamp":1785196800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T00:00:00Z","timestamp":1785196800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100023581","name":"National Science Foundation Graduate Research Fellowship Program","doi-asserted-by":"publisher","award":["DGE 2040434"],"award-info":[{"award-number":["DGE 2040434"]}],"id":[{"id":"10.13039\/100023581","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 DK120921"],"award-info":[{"award-number":["R01 DK120921"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 HL171197"],"award-info":[{"award-number":["R01 HL171197"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["F32 AR084876"],"award-info":[{"award-number":["F32 AR084876"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1S10OD034320"],"award-info":[{"award-number":["1S10OD034320"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Materials"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Granular hydrogels offer a powerful platform for engineering porous, cell\u2010instructive scaffolds with tunable mechanical, structural, and biochemical properties, yet introducing spatial and functional heterogeneity typically requires multiple microgel populations or complex fabrication strategies. Here, we present a programmable, photoresponsive granular hydrogel platform that enables post\u2010assembly spatiotemporal control of scaffold mechanics and cell microenvironments from a single microgel formulation. Poly(ethylene glycol) microgels containing photolabile allyl sulfide moieties were synthesized via strain\u2010promoted azide\u2010alkyne cycloaddition and assembled into granular scaffolds capable of light\u2010mediated remodeling through radical addition\u2010fragmentation chain transfer. This chemistry afforded dynamic, on\u2010demand, and spatially defined tuning of mechanical properties (G\u2032 = 0.7\u20133.7\u00a0kPa) while maintaining scaffold porosity (\u223c20%). High\u2010resolution photopatterning across multiple length (6\u00a0\u00b5m\u20101\u00a0mm) and timescales enabled precise modulation of local microenvironments. Human mesenchymal stem\/stromal cells embedded in these scaffolds responded to spatiotemporal modulation of matrix mechanics as observed by changes in morphology, yes\u2010associated protein 1 (YAP) nuclear localization, and secretory profiles. Together, these results establish a versatile and broadly applicable strategy for programming mechanical heterogeneity and regulating cell behavior in granular hydrogels through photolabile moieties.<\/jats:p>","DOI":"10.1002\/adma.74258","type":"journal-article","created":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T12:45:01Z","timestamp":1785242701000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Photoresponsive Granular Hydrogels Enable Spatiotemporal Control of Matrix Mechanics and MSC Behavior"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5090-6538","authenticated-orcid":false,"given":"Nicole E.","family":"Friend","sequence":"first","affiliation":[{"name":"Department of Chemical and Biological Engineering University of Colorado Boulder  Boulder Colorado USA"},{"name":"BioFrontiers Institute University of Colorado Boulder  Boulder Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7064-5466","authenticated-orcid":false,"given":"Nolan R.","family":"Petrich","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering University of Colorado Boulder  Boulder Colorado USA"},{"name":"BioFrontiers Institute University of Colorado Boulder  Boulder Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kara E.","family":"Shockley","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular, and Developmental Biology University of Colorado Boulder  Boulder Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-6939-0655","authenticated-orcid":false,"given":"Ashbey N.","family":"Manning","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering University of Colorado Boulder  Boulder Colorado USA"},{"name":"BioFrontiers Institute University of Colorado Boulder  Boulder Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew W.","family":"Jaeschke","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering University of Colorado Boulder  Boulder Colorado USA"},{"name":"BioFrontiers Institute University of Colorado Boulder  Boulder Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Saeb","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering University of Colorado Boulder  Boulder Colorado USA"},{"name":"BioFrontiers Institute University of Colorado Boulder  Boulder Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark W.","family":"Young","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering University of Colorado Boulder  Boulder Colorado USA"},{"name":"BioFrontiers Institute University of Colorado Boulder  Boulder Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5725-5691","authenticated-orcid":false,"given":"Kristi S.","family":"Anseth","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering University of Colorado Boulder  Boulder Colorado USA"},{"name":"BioFrontiers Institute University of Colorado Boulder  Boulder Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,28]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmat4294"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.0c01612"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbiosy.2021.100008"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201907670"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2026.02.030"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202501947"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2019.02.054"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202202239"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202512971"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2019.119725"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2022.04.023"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202307928"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/admi.202401037"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202300823"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202109194"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202511513"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2025.12.040"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202310507"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201900071"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202200951"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202106896"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2019.11.009"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201907102"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1039\/d4bm00233d"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2022.07.051"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.4c02247"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/btm2.10217"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202400249"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202201921"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2023.03.045"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202400567"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202201122"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1039\/d3bm00721a"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2025.02.003"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2025.06.006"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1039\/d3lp00249g"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201605001"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202413778"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201901214"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201905366"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.add5668"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celbio.2025.100046"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202109252"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.8b07551"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.3390\/polym12071489"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/app.42061"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.4983048"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1039\/c5bm00034c"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox12051094"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox8040082"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.1c01440"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apt.2023.104106"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eurpolymj.2026.114647"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43588\u2010023\u201000551\u2010x"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.accounts.4c00548"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchem.1174"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscentsci.8b00325"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1169494"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.200900917"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1063\/5.0077004"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202501585"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2015.05.010"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201054577\u20104"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2021571118"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201059174\u20101"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.4252\/wjsc.v6.i5.552"},{"key":"e_1_2_8_68_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology15100794"},{"key":"e_1_2_8_69_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202001948"},{"key":"e_1_2_8_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.06.044"},{"key":"e_1_2_8_71_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10137"},{"key":"e_1_2_8_72_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2017.87"},{"key":"e_1_2_8_73_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41409\u2010022\u201001728\u20100"},{"key":"e_1_2_8_74_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0903269107"},{"key":"e_1_2_8_75_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.3c00954"},{"key":"e_1_2_8_76_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2020.0030"},{"key":"e_1_2_8_77_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202403198"},{"key":"e_1_2_8_78_1","doi-asserted-by":"publisher","DOI":"10.1063\/5.0013707"},{"key":"e_1_2_8_79_1","doi-asserted-by":"publisher","DOI":"10.1364\/OE.496888"},{"key":"e_1_2_8_80_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.229013"},{"key":"e_1_2_8_81_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202211209"},{"key":"e_1_2_8_82_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202302925"},{"key":"e_1_2_8_83_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.201800638"}],"container-title":["Advanced Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adma.74258","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/adma.74258","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adma.74258","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T12:33:33Z","timestamp":1790858013000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adma.74258"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,28]]},"references-count":82,"journal-issue":{"issue":"55","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1002\/adma.74258"],"URL":"https:\/\/doi.org\/10.1002\/adma.74258","archive":["Portico"],"relation":{},"ISSN":["0935-9648","1521-4095"],"issn-type":[{"value":"0935-9648","type":"print"},{"value":"1521-4095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,28]]},"assertion":[{"value":"2026-04-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e74258"}}