{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T08:14:40Z","timestamp":1790151280571,"version":"4.0.1"},"reference-count":53,"publisher":"Wiley","issue":"52","license":[{"start":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:00:00Z","timestamp":1785456000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:00:00Z","timestamp":1785456000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100014523","name":"Institute for Critical Technology and Applied Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014523","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000150","name":"Office of Emerging Frontiers and Multidisciplinary Activities","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000150","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Materials"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Autogenic engineered living materials (ELMs) enable the in situ production and engineering of native extracellular matrix (ECM). However, existing autogenic ELMs remain limited in scope and functionality. Here, we present a versatile platform for\n                    <jats:italic>de novo<\/jats:italic>\n                    autogenic functional ELMs, leveraging protein mining, computational modeling, and synthetic biology. By analyzing 33,564 CsgA\u2010like homologs, we identify candidates for\n                    <jats:italic>de novo<\/jats:italic>\n                    ECM protein nanofibers. Using AlphaFold2 and molecular dynamics simulations, we elucidate the structural stability of these \u03b2\u2010solenoid proteins. By reprogramming the\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    curli machinery, we achieve the biosynthesis of CsgA\u2010like ELMs from non\u2010model bacteria, featuring up to a 9\u2010fold increased molecular weight and expanded \u03b2\u2010sheet repeat units. Furthermore, we fabricate macroscopic biomaterials with enhanced mechanical properties (a 3\u2010fold increase in storage modulus), and their extracellular fiber networks attenuate UV\u2010C irradiation, extending the survival of embedded cells by 5\u2010fold. We further demonstrate programmable functionalities, including 3D printability and selective binding to nanoparticles and antibodies. This work establishes a powerful framework for discovering, designing, and harnessing natural biomolecular systems to advance next\u2010generation autogenic ELMs.\n                  <\/jats:p>","DOI":"10.1002\/adma.74312","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T14:32:27Z","timestamp":1785508347000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>De Novo<\/i>\n                    Autogenic Engineered Living Functional Materials"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-6311-7993","authenticated-orcid":false,"given":"Hoda M.","family":"Hammad","sequence":"first","affiliation":[{"name":"Department of Biological Systems Engineering Virginia Tech  Blacksburg Virginia 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Virginia USA"},{"name":"Department of Chemistry Virginia Tech  Blacksburg Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanket","family":"Deshmukh","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Virginia Tech  Blacksburg Virginia USA"},{"name":"Macromolecules Innovation Institute Virginia Tech  Blacksburg Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7806-2015","authenticated-orcid":false,"given":"Avinash","family":"Manjula\u2010Basavanna","sequence":"additional","affiliation":[{"name":"Department of Biological Systems Engineering Virginia Tech  Blacksburg Virginia USA"},{"name":"Department of Chemistry and Chemical Biology Northeastern University  Boston Massachusetts USA"},{"name":"Department of Bioengineering Northeastern University  Boston Massachusetts 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