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Here, we demonstrate that micro\u2010topographical cues applied during initial IEO formation enhance the development and functional maturation of PSC\u2010derived IEOs. This microengineering strategy introduces temporally defined microscale geometric confinement to regulate early cell\u2010cell and cell\u2010extracellular matrix (ECM) interactions, thereby promoting epithelial organization and developmental fidelity. Microengineered IEOs (M\u2010IEOs) exhibit improved reproducibility and neurosensory maturation, including increased hair cell\u2010like populations, stereocilia\u2010like structures and kinocilium\u2010like features exhibiting a characteristic (9 \u00d7 2) + 2 microtubule organization. Functionally, M\u2010IEOs exhibit enhanced electrophysiological responsiveness, supported by complementary transcriptomic and in situ analyses indicating activation of inner ear lineage maturation pathways. Furthermore, we demonstrate the versatility of M\u2010IEOs by integrating them with a microfluidic vascular system to model vascular\u2010epithelial interactions and inflammatory responses, highlighting its potential for disease modeling and pharmacological screening. Together, these findings establish transient micro\u2010topographical guidance as an instructive regulator of inner ear organoid development and provide a robust, vascular\u2010compatible platform for neurosensory research, disease modeling, and translational screening applications.<\/jats:p>","DOI":"10.1002\/adma.74560","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T06:04:36Z","timestamp":1786428276000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Engineering Pluripotent Stem Cells\u2010Derived Inner Ear Organoids With Enhanced Maturation and Reproducibility by Micro\u2010Topographical Cues"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5986-9294","authenticated-orcid":false,"given":"Harshita","family":"Sharma","sequence":"first","affiliation":[{"name":"Department of Convergence Biosystems Engineering Chonnam National University  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