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Optogenetics offers versatile potential for non\u2010invasive regulation of biological processes. Yet, its integration within large\u2010scale, cell\u2010laden bioprinted materials is still limited, especially considering the spatial constraints of existing light delivery methods. In this study, we introduce a novel approach that repurposes tomographic volumetric bioprinting to enable post\u2010printing stimulation of photosensitive protein\u2010switches and optogenetic circuits in cells deep within hydrogel constructs. By converging different bioprinting approaches, computer vision, context\u2010aware model generation, and synthetic biology and cell engineering, we demonstrated selective activation of a fluorescent, light\u2010responsive protein probe within multi\u2010material centimeter\u2010scale constructs. Moreover, leveraging a multi\u2010wavelength volumetric bioprinter, we further demonstrate this concept by selectively stimulating cells expressing a near\u2010infrared optogenetic system that triggers gene expression and the induction of pancreas\u2010specific transcription factors. The described methods provide platforms for remote, repeatable, and localized control of biological events in volumetric constructs, opening new possibilities for advanced tissue models, and dynamic tuning of cell\u2010mediated protein production in engineered living systems.<\/jats:p>","DOI":"10.1002\/adma.74563","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T12:24:21Z","timestamp":1786364661000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Spatial Control of Light\u2010Responsive Proteins and Optogenetics Within Hydrogels via Volumetric Bioprinting"],"prefix":"10.1002","author":[{"given":"Davide","family":"Ribezzi","sequence":"first","affiliation":[{"name":"Regenerative Medicine Center Utrecht University Medical Center Utrecht Utrecht University  Utrecht The Netherlands"},{"name":"Department of Orthopaedics University Medical Center Utrecht Utrecht University  Utrecht 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