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RNA\u2010cleaving DNAzymes offer a promising route to accelerate bacterial diagnosis because they can be selected and programmed to specifically recognize disease\u2010causing bacteria and generate reporter DNA strands for signal readout. However, integrating DNAzymes into biosensors capable of analyzing complex biological matrices remains challenging, as matrix\u2010associated interferents can destabilize DNAzymes, suppress catalytic activity, and compromise signal detection. Here, we integrate redox RNA\u2010cleaving DNAzymes housed on antifouling magnetic beads with a lab\u2010in\u2010a\u2010tube platform that incorporates hierarchically structured sensing electrodes within a tubular flow cell for enrichment\u2010free detection of\n                    <jats:italic>Clostridioides difficile<\/jats:italic>\n                    in stool samples. The combination of antifouling magnetic beads and fluidic replenishment of DNA reporters across the hierarchical electrodes reduces nonspecific binding and enhances reporter capture efficiency, achieving a limit of detection as low as 1.3\u00a0\u00d7\u00a010\n                    <jats:sup>2<\/jats:sup>\n                    CFU mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    in buffer. Using an optimized stool\u2010processing procedure, the sensor enabled detection of\n                    <jats:italic>C. difficile<\/jats:italic>\n                    infection in 38 human stool samples, demonstrating 94.7% concordance with the current gold\u2010standard polymerase chain reaction method. These results demonstrate a promising platform for enrichment\u2010free, point\u2010of\u2010care diagnosis of infectious diseases in clinic settings.\n                  <\/jats:p>","DOI":"10.1002\/adma.74907","type":"journal-article","created":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T17:08:55Z","timestamp":1788628135000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Lab\u2010in\u2010a\u2010Tube System Integrating Hierarchically Wrinkled Electrodes and DNAzyme\u2010Functionalized Beads for Diagnosis of\n                    <i>Clostridioides difficile<\/i>\n                    Infection"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2333-7215","authenticated-orcid":false,"given":"Survanshu","family":"Saxena","sequence":"first","affiliation":[{"name":"School of Biomedical Engineering McMaster University  Hamilton Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0109-6285","authenticated-orcid":false,"given":"Soyeon","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biomedical Sciences McMaster University  Hamilton Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4442-4898","authenticated-orcid":false,"given":"Youngeun","family":"Choi","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering McMaster University  Hamilton Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"L'Heureux\u2010Hache","sequence":"additional","affiliation":[{"name":"Department of Engineering Physics McMaster University  Hamilton Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-0966-5865","authenticated-orcid":false,"given":"Jiaying","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biomedical Sciences McMaster University  Hamilton Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7601-6680","authenticated-orcid":false,"given":"Sadman","family":"Sakib","sequence":"additional","affiliation":[{"name":"Department of Engineering Physics McMaster University  Hamilton Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara M.","family":"Imani","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering McMaster University  Hamilton Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1655-3761","authenticated-orcid":false,"given":"Marek","family":"Smieja","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Medicine McMaster University  Hamilton Canada"},{"name":"Michael G. DeGroote Institute for Infectious Disease Research McMaster University  Hamilton Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7533-6743","authenticated-orcid":false,"given":"Yingfu","family":"Li","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering McMaster University  Hamilton Canada"},{"name":"Department of Biochemistry and Biomedical Sciences McMaster University  Hamilton Canada"},{"name":"Michael G. 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