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However, current limitations include reproducibility issues largely due to the use of biologically derived materials, which are prone to batch\u2010to\u2010batch variations. Here, we report a chemically defined microgel\u2010based method for human induced pluripotent stem cell (iPSC)\u2010based organoid generation, enabling expansion of iPSCs and their subsequent differentiation within one construct across different scales, including compatibility with automated 384\u2010well plate workflows. Chemically defined poly(ethylene glycol) (PEG)\u2010based microgels are produced via parallelized step\u2010emulsification microfluidics, enabling scalable production. This approach leverages the self\u2010organization of iPSCs with microgels to build three\u2010dimensional constructs, driven by robust cell\u2010material interactions achieved through vitronectin\u2010coated PEG microgels. This technology allows the iPSCs to expand and retain their pluripotency, after which they can be differentiated into the three germ layers, providing a suitable platform for organoid differentiation. This was further extended by differentiation into cardiac organoids and retinal photoreceptors to demonstrate two exemplary tissues.<\/jats:p>","DOI":"10.1002\/adma.75116","type":"journal-article","created":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T13:47:26Z","timestamp":1790171246000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Microgels Enable iPSCs to Assemble, Expand, and Differentiate Into Organoids\u2014From Sizable to High\u2010Throughput"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-0136-680X","authenticated-orcid":false,"given":"Laura","family":"Klasen","sequence":"first","affiliation":[{"name":"Institute For Technical and Macromolecular Chemistry RWTH Aachen University  Aachen Germany"},{"name":"DWI\u2014Leibniz Institute for Interactive Materials e.V.  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Aachen Germany"},{"name":"Department of Advanced Materials For Biomedicine (AMB) CBMS\u2014Center For Biohybrid Medical Systems AME\u2014Institute of Applied Medical Engineering University Hospital RWTH Aachen  Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,23]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.150201"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature22796"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3275"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12271"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010021\u201000815\u20109"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010021\u201025329\u20105"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010023\u201043999\u20101"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4304"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature22330"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12517"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09941"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1324212111"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2012.175"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2023.04.011"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0905550106"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2021.01.026"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201700980"},{"key":"e_1_2_8_19_1","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1007\/978-3-030-93056-1_8","volume-title":"Organoid Technology for Disease Modelling and Personalized Treatment","author":"Abdul Samat A.","year":"2022"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200900758"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.143933"},{"key":"e_1_2_8_22_1","article-title":"Development of Collagen\u2010Based 3D Matrix for Gastrointestinal Tract\u2010Derived Organoid Culture","volume":"1","author":"Jee J. 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