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Saliva is considered as an alternative specimen to detect the severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102) since the viral load is comparable to what are found in a throat and a nasal cavity. The electrical double layer (EDL)\u2010gated field\u2010effect transistor\u2010based biosensor (BioFET) emerges as a promising candidate for salivary COVID\u201019 tests due to a high sensitivity, a portable configuration, a label\u2010free operation, and a matrix insensitivity. In this work, the authors utilize EDL\u2010gated BioFETs to detect complementary DNAs (cDNAs) and viral RNAs with various testing conditions such as switches of probes, temperature treatments, and matrices. The selectivity is confirmed with cDNA and noncomplementary DNA (ncDNA), exhibiting an eightfold difference in electrical signals. The matrix insensitivity is evaluated, and BioFETs successfully validate the detection of SARS\u2010CoV\u20102 N\u2010gene RNA down to 1 f<jats:sc>m<\/jats:sc> in diluted human saliva with a 95\u00b0C\u2010 and a 25\u00b0C\u2010treatment, respectively. This proposed system has a high potential to be deployed for an on\u2010site COVID\u201019 screening, improving the disease control and benefitting frontline healthcare system.<\/jats:p>","DOI":"10.1002\/admt.202100842","type":"journal-article","created":{"date-parts":[[2021,9,12]],"date-time":"2021-09-12T14:30:59Z","timestamp":1631457059000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["A Rapid Detection of COVID\u201019 Viral RNA in Human Saliva Using Electrical Double Layer\u2010Gated Field\u2010Effect Transistor\u2010Based Biosensors"],"prefix":"10.1002","volume":"7","author":[{"given":"Akhil K.","family":"Paulose","sequence":"first","affiliation":[{"name":"Institute of Nanoengineering and Microsystems National Tsing Hua University  Hsinchu 30013 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0972-6444","authenticated-orcid":false,"given":"Chih\u2010Cheng","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute of Nanoengineering and Microsystems National Tsing Hua University  Hsinchu 30013 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Po\u2010Hsuan","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Nanoengineering and Microsystems National Tsing Hua University  Hsinchu 30013 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adarsh","family":"Tripathi","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine National Tsing Hua University  Hsinchu 30013 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pin\u2010Hsuan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Power Mechanical Engineering National Tsing Hua University  Hsinchu 30013 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010Shan","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute of Nanoengineering and Microsystems National Tsing Hua University  Hsinchu 30013 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4791-936X","authenticated-orcid":false,"given":"Yu\u2010Lin","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Nanoengineering and Microsystems National Tsing Hua University  Hsinchu 30013 Taiwan"},{"name":"Department of Power Mechanical Engineering National Tsing Hua University  Hsinchu 30013 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,9,12]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30185-9"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2012-7"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.45053"},{"key":"e_1_2_8_4_1","unstructured":"World Health Organization Weekly epidemiological update on COVID\u201019 https:\/\/www.who.int\/docs\/default-source\/coronaviruse\/situation-reports\/20210622_weekly_epi_update_45.pdf(accessed: June2021)."},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10544-020-00534-z"},{"key":"e_1_2_8_6_1","first-page":"69","volume":"38","author":"Kanjanaumporn J.","year":"2020","journal-title":"Asian Pac. 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