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Annealing at temperatures of 80, 100, 120, and 140\u00a0\u00b0C is assessed for its impact on material properties and physiological utility. Analytical techniques such as Differential Scanning Calorimetry (DSC), Fourier\u2010Transform Infrared Spectroscopy (FTIR), and X\u2010ray Diffraction (XRD) reveal increased crystallinity with higher annealing temperatures, peaking in \u03b2\u2010phase content and crystallinity at 140\u00a0\u00b0C. Scanning Electron Microscopy (SEM) shows that 140\u00a0\u00b0C annealed scaffolds have enhanced lamellar structures, increased porosity, and maximum piezoelectric response. Mechanical tests indicate that 140\u00a0\u00b0C annealing improved elastic modulus, tensile strength, and substrate stiffness, aligning these properties with physiological soft tissues. In vitro assessments in Schwann cells demonstrate favorable responses, with increased cell proliferation, contraction, and extracellular matrix attachment. Additionally, genes linked to extracellular matrix production, vascularization, and calcium signaling are upregulated. The foreign body response in C57BL\/6 mice, evaluated through Hematoxylin and Eosin (H&amp;E) and Picrosirius Red staining, shows no differences between scaffold groups, supporting the potential for future functional evaluation of the annealed group in tissue repair.<\/jats:p>","DOI":"10.1002\/admt.202401513","type":"journal-article","created":{"date-parts":[[2025,7,10]],"date-time":"2025-07-10T02:45:35Z","timestamp":1752115535000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Thermal Annealing Enhances Piezoelectricity and Regenerative Potential of PVDF\u2010TrFE Nanofiber Scaffolds"],"prefix":"10.1002","volume":"10","author":[{"given":"Maksym","family":"Krutko","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering University of Cincinnati  Cincinnati OH 45221 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holly M.","family":"Poling","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Cincinnati  Cincinnati OH 45221 USA"},{"name":"Center for Stem Cell and Organoid Medicine (CuSTOM) Cincinnati Children's Hospital Medical Center  Cincinnati OH 45229 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maulee","family":"Sheth","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Cincinnati  Cincinnati OH 45221 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Supasek","family":"Kongsomros","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Cincinnati  Cincinnati OH 45221 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew E.","family":"Bryan","sequence":"additional","affiliation":[{"name":"Department of Chemical &amp; 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